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AzoDye-3 Azide

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Physical properties
Molecular weight651.22
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
651.22
AzoDye-3 azide is an excellent building block for labeling alkyne-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM153.558 µL767.79 µL1.536 mL7.678 mL15.356 mL
5 mM30.712 µL153.558 µL307.116 µL1.536 mL3.071 mL
10 mM15.356 µL76.779 µL153.558 µL767.79 µL1.536 mL

Molarity calculator

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Spectrum


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References


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