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AzoDye-3 CPG (500 A)

AzoDye-3 CPG is an excellent building block for preparing FRET oligo molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

References

View all 50 references: Citation Explorer
Hairpin oligosensor using SiQDs: Förster resonance energy transfer study and application for miRNA-21 detection.
Authors: Mahani, Mohamad and Khakbaz, Faeze and Ju, Huangxian
Journal: Analytical and bioanalytical chemistry (2022): 2505-2512
FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2.
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143
Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.
Authors: Zhang, Hanwen and Zhang, Yuchen and Xu, Wenting and Li, Rong and Zhang, Dabing and Yang, Litao
Journal: Food chemistry. Molecular sciences (2022): 100061
Multivalent self-assembled nano string lights for tumor-targeted delivery and accelerated biomarker imaging in living cells and in vivo.
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818
Broad-Spectrum Polymeric Nanoquencher as an Efficient Fluorescence Sensing Platform for Biomolecular Detection.
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111
Page updated on October 22, 2024

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Physical properties

Molecular weight

N/A

Solvent

MeCN

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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