AzoDye-3 CPG (500 A)

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Physical properties

Molecular weight	N/A
Solvent	MeCN

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

N/A

AzoDye-3 CPG is an excellent building block for preparing FRET oligo molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

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Spectral properties

Images

Figure 1. AzoDye-3 CPG is an excellent building block for preparing FRET oligo molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients.

References

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Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143

Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.
Authors: Zhang, Hanwen and Zhang, Yuchen and Xu, Wenting and Li, Rong and Zhang, Dabing and Yang, Litao
Journal: Food chemistry. Molecular sciences (2022): 100061

Multivalent self-assembled nano string lights for tumor-targeted delivery and accelerated biomarker imaging in living cells and in vivo.
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818

Double-PEGylated Cyclopeptidic Photosensitizer Prodrug Improves Drug Uptake from In Vitro to Hen's Egg Chorioallantoic Membrane Model.
Authors: Bouilloux, Jordan and Kiening, Martin and Yapi, Sopie and Lange, Norbert
Journal: Molecules (Basel, Switzerland) (2021)

Hyaluronic Acid Derived Hypoxia-Sensitive Nanocarrier for Tumor Targeted Drug Delivery.
Authors: Le, Trong-Nghia and Lin, Chin-Jung and Shen, Yen Chen and Lin, Kuan-Yu and Lee, Cheng-Kang and Huang, Chih-Ching and Rao, N Vijayakameswara
Journal: ACS applied bio materials (2021): 8325-8332

DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763

Broad-Spectrum Polymeric Nanoquencher as an Efficient Fluorescence Sensing Platform for Biomolecular Detection.
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111

Targeted Delivery and Site-Specific Activation of β-Cyclodextrin-Conjugated Photosensitizers for Photodynamic Therapy through a Supramolecular Bio-orthogonal Approach.
Authors: Xue, Evelyn Y and Shi, Wen-Jing and Fong, Wing-Ping and Ng, Dennis K P
Journal: Journal of medicinal chemistry (2021): 15461-15476

Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352