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AzoDye-3 PEG10 Alkyne

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Physical properties
Molecular weight1138.29
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
AzoDye-3 PEG10 Azide

OverviewpdfSDSpdfProtocol


Molecular weight
1138.29
AzoDye-3 PEG10 alkyne is an excellent building block for labeling azide-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-3 alkyne (#2484) although both AzoDye-3 alkyne (#2484) and AzoDye-3 PEG10 alkyne (#2485) bear the same extremely hydrophobic BHQ-2 chromophore. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 PEG10 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.851 µL439.255 µL878.511 µL4.393 mL8.785 mL
5 mM17.57 µL87.851 µL175.702 µL878.511 µL1.757 mL
10 mM8.785 µL43.926 µL87.851 µL439.255 µL878.511 µL

Molarity calculator

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Spectrum


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References


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