BAPTA, AM *CAS 126150-97-8*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 764.68 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Alternative formats
| BAPTA, AM *UltraPure Grade* *CAS 126150-97-8* |
| Overview |  SDSProtocol |
See also: Calcium Indicators
CAS 126150-97-8 | Molecular weight 764.68 |
BAPTA AM is cell-permeable version of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), a cell-permeable calcium chelator. This BAPTA derivative is used for adjusting calcium concentrations in cells and tissues. BAPTA is a calcium-specific aminopolycarboxylic acid. The presence of four carboxylic acid functional groups makes possible the binding of two calcium ions. The extensive flexibility of the carboxylate ligands is critical to the coordination of calcium and other metal ions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BAPTA, AM *CAS 126150-97-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 130.774 µL | 653.868 µL | 1.308 mL | 6.539 mL | 13.077 mL |
| 5 mM | 26.155 µL | 130.774 µL | 261.547 µL | 1.308 mL | 2.615 mL |
| 10 mM | 13.077 µL | 65.387 µL | 130.774 µL | 653.868 µL | 1.308 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) |
| BCECF, AM | 504 | 527 |
| BCECF, AM *UltraPure grade* | 504 | 527 |
| Zinquin AM *UltraPure grade* | 355 | 491 |
| Calcein, AM *CAS 148504-34-1* | 501 | 521 |
| Calcein, AM *UltraPure grade* *CAS 148504-34-1* | 501 | 521 |
Images
Figure 1. Chemical structure for BAPTA, AM *CAS 126150-97-8*
Citations
View all 10 citations: Citation Explorer
RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether
Authors: Liao, Ya-Cheng and Fernandopulle, Michael S and Wang, Guozhen and Choi, Heejun and Hao, Ling and Drerup, Catherine M and Patel, Rajan and Qamar, Seema and Nixon-Abell, Jonathon and Shen, Yi and others,
Journal: Cell (2019): 147--164
Authors: Liao, Ya-Cheng and Fernandopulle, Michael S and Wang, Guozhen and Choi, Heejun and Hao, Ling and Drerup, Catherine M and Patel, Rajan and Qamar, Seema and Nixon-Abell, Jonathon and Shen, Yi and others,
Journal: Cell (2019): 147--164
The role of calcium signalling in autophagy
Authors: Chehab, Tala
Journal: (2018)
Authors: Chehab, Tala
Journal: (2018)
Angiotensin II regulates phosphorylation of actin-associated proteins in human podocytes
Authors: Schenk, Laura K and M{\"o}ller-Kerutt, Annika and Klosowski, Rafael and Wolters, Dirk and Schaffner-Reckinger, Elisabeth and Weide, Thomas and Pavenst{\"a}dt, Hermann and Vollenbr{\"o}ker, Beate
Journal: The FASEB Journal (2017): 5019--5035
Authors: Schenk, Laura K and M{\"o}ller-Kerutt, Annika and Klosowski, Rafael and Wolters, Dirk and Schaffner-Reckinger, Elisabeth and Weide, Thomas and Pavenst{\"a}dt, Hermann and Vollenbr{\"o}ker, Beate
Journal: The FASEB Journal (2017): 5019--5035
Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
Helicobacter pylori induces IL-1β protein through the inflammasome activation in differentiated macrophagic cells
Authors: Kameoka, Shoichiro and Kameyama, Takeshi and Hayashi, Takaya and Sato, Seiichi and Ohnishi, Naomi and Hayashi, Takeru and Murata-Kamiya, Naoko and Higashi, Hideaki and Hatakeyama, Masanori and Takaoka, Akinori
Journal: Biomedical Research (2016): 21--27
Authors: Kameoka, Shoichiro and Kameyama, Takeshi and Hayashi, Takaya and Sato, Seiichi and Ohnishi, Naomi and Hayashi, Takeru and Murata-Kamiya, Naoko and Higashi, Hideaki and Hatakeyama, Masanori and Takaoka, Akinori
Journal: Biomedical Research (2016): 21--27
Spautin-1 Ameliorates Acute Pancreatitis via Inhibiting Impaired Autophagy and Alleviating Calcium Overload
Authors: Xiao, Juan and Feng, Xueping and Huang, Xiao-Ying and Huang, Zhongshi and Huang, Yanqiang and Li, Chaogan and Li, Genliang and Nong, Song and Wu, Ruoshi and Huang, Yongzhi and others, undefined
Journal: Molecular Medicine (2016): 643
Authors: Xiao, Juan and Feng, Xueping and Huang, Xiao-Ying and Huang, Zhongshi and Huang, Yanqiang and Li, Chaogan and Li, Genliang and Nong, Song and Wu, Ruoshi and Huang, Yongzhi and others, undefined
Journal: Molecular Medicine (2016): 643
Role of Secretory Processes in Cardiac Fibroblasts for Heart Failure Development and Progression
Authors: Kittana, Naim
Journal: (2014)
Authors: Kittana, Naim
Journal: (2014)
Ras guanyl nucleotide releasing protein 2 affects cell viability and cell--matrix adhesion in ECV304 endothelial cells
Authors: Takino, Junichi and Nagamine, Kentaro and Hori, Takamitsu
Journal: Cell adhesion & migration (2013): 262--266
Authors: Takino, Junichi and Nagamine, Kentaro and Hori, Takamitsu
Journal: Cell adhesion & migration (2013): 262--266
Delayed administration of the nucleic acid analog 2Cl-C. OXT-A attenuates brain damage and enhances functional recovery after ischemic stroke
Authors: Okabe, Naohiko and Nakamura, Emi and Himi, Naoyuki and Narita, Kazuhiko and Tsukamoto, Ikuko and Maruyama, Tokumi and Sakakibara, Norikazu and Nakamura, Takehiro and Itano, Toshifumi and Miyamoto, Osamu
Journal: Brain research (2013): 115--131
Authors: Okabe, Naohiko and Nakamura, Emi and Himi, Naoyuki and Narita, Kazuhiko and Tsukamoto, Ikuko and Maruyama, Tokumi and Sakakibara, Norikazu and Nakamura, Takehiro and Itano, Toshifumi and Miyamoto, Osamu
Journal: Brain research (2013): 115--131
Involvement of thioredoxin-binding protein 2 in the antitumor activity of CD437
Authors: Matsuoka, Saori and Tsuchiya, Hiroyuki and Sakabe, Tomohiko and Watanabe, Yumi and Hoshikawa, Yoshiko and Kurimasa, Akihiro and Itamochi, Hiroaki and Harada, Tasuku and Terakawa, Naoki and Masutani, Hiroshi and others, undefined
Journal: Cancer science (2008): 2485--2490
Authors: Matsuoka, Saori and Tsuchiya, Hiroyuki and Sakabe, Tomohiko and Watanabe, Yumi and Hoshikawa, Yoshiko and Kurimasa, Akihiro and Itamochi, Hiroaki and Harada, Tasuku and Terakawa, Naoki and Masutani, Hiroshi and others, undefined
Journal: Cancer science (2008): 2485--2490
References
View all 43 references: Citation Explorer
Determination of BAPTA-AM, the acetoxymethyl tetraester of BAPTA, in rat plasma by liquid chromatography tandem mass spectrometry
Authors: Zheng F, Hang T, Wu C, Di B, Liu W, Xu J, Zhai C, Sun D, Liu J, Wei W.
Journal: J Mass Spectrom (2006): 1615
Authors: Zheng F, Hang T, Wu C, Di B, Liu W, Xu J, Zhai C, Sun D, Liu J, Wei W.
Journal: J Mass Spectrom (2006): 1615
Abrogation of DTH response and mitogenic lectin- and alloantigen-induced activation of lymphocytes by calcium inhibitors TMB-8 and BAPTA-AM
Authors: Saxena HM, Dikshit M.
Journal: Immunol Lett (2005): 60
Authors: Saxena HM, Dikshit M.
Journal: Immunol Lett (2005): 60
BAPTA induces frequency shifts in vivo of spontaneous otoacoustic emissions of the bobtail lizard
Authors: Manley GA, Kirk DL.
Journal: Audiol Neurootol (2005): 248
Authors: Manley GA, Kirk DL.
Journal: Audiol Neurootol (2005): 248
Ca2+-dependent interaction of BAPTA with phospholipids
Authors: Rousset M, Cens T, Vanmau N, Charnet P.
Journal: FEBS Lett (2004): 41
Authors: Rousset M, Cens T, Vanmau N, Charnet P.
Journal: FEBS Lett (2004): 41
Calcium-independent cytoskeleton disassembly induced by BAPTA
Authors: Saoudi Y, Rousseau B, Doussiere J, Charrasse S, Gauthier-Rouviere C, Morin N, Sautet-Laugier C, Denarier E, Scaife R, Mioskowski C, Job D.
Journal: Eur J Biochem (2004): 3255
Authors: Saoudi Y, Rousseau B, Doussiere J, Charrasse S, Gauthier-Rouviere C, Morin N, Sautet-Laugier C, Denarier E, Scaife R, Mioskowski C, Job D.
Journal: Eur J Biochem (2004): 3255
Disturbed cooperation of hepatocytes in the rhythm of protein synthesis by chelating agent of cytoplasmic calcium BAPTA-AM
Authors: Zvesdina ND, Nechaeva NV, Gracheva EV, Novikova TE, Gvazava IG, Fateeva VI, Mal'chenko LA, Brodskii V.
Journal: Izv Akad Nauk Ser Biol (2003): 14
Authors: Zvesdina ND, Nechaeva NV, Gracheva EV, Novikova TE, Gvazava IG, Fateeva VI, Mal'chenko LA, Brodskii V.
Journal: Izv Akad Nauk Ser Biol (2003): 14
Loading neurons with BAPTA-AM activates xbp1 processing indicative of induction of endoplasmic reticulum stress
Authors: Paschen W, Hotop S, Aufenberg C.
Journal: Cell Calcium (2003): 83
Authors: Paschen W, Hotop S, Aufenberg C.
Journal: Cell Calcium (2003): 83
Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151
BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures
Authors: Wie MB, Koh JY, Won MH, Lee JC, Shin TK, Moon CJ, Ha HJ, Park SM, Kim HC.
Journal: Prog Neuropsychopharmacol Biol Psychiatry (2001): 1641
Authors: Wie MB, Koh JY, Won MH, Lee JC, Shin TK, Moon CJ, Ha HJ, Park SM, Kim HC.
Journal: Prog Neuropsychopharmacol Biol Psychiatry (2001): 1641
The calcium chelator BAPTA affects the binding of assembly protein AP-2 to membranes
Authors: Alberdi A, Jimenez-Ortiz V, Sosa MA.
Journal: Biocell (2001): 167
Authors: Alberdi A, Jimenez-Ortiz V, Sosa MA.
Journal: Biocell (2001): 167
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Does EDTA inactivate proteinase K?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Does EDTA inactivate proteinase K?