Calculator
BAPTA, AM *UltraPure Grade* *CAS 126150-97-8*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 764.68 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Alternative formats
| BAPTA, AM *CAS 126150-97-8* |
| Overview |  SDSProtocol |
See also: Calcium Indicators
CAS 126150-97-8 | Molecular weight 764.68 |
BAPTA AM is cell-permeable version of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), a cell-permeable calcium chelator. This BAPTA derivative is used for adjusting calcium concentrations in cells and tissues. BAPTA is a calcium-specific aminopolycarboxylic acid. The presence of four carboxylic acid functional groups makes possible the binding of two calcium ions. The extensive flexibility of the carboxylate ligands is critical to the coordination of calcium and other metal ions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BAPTA, AM *UltraPure Grade* *CAS 126150-97-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 130.774 µL | 653.868 µL | 1.308 mL | 6.539 mL | 13.077 mL |
| 5 mM | 26.155 µL | 130.774 µL | 261.547 µL | 1.308 mL | 2.615 mL |
| 10 mM | 13.077 µL | 65.387 µL | 130.774 µL | 653.868 µL | 1.308 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) |
| BCECF, AM | 504 | 527 |
| BCECF, AM *UltraPure grade* | 504 | 527 |
| Zinquin AM *UltraPure grade* | 355 | 491 |
| Calcein, AM *CAS 148504-34-1* | 501 | 521 |
| Calcein, AM *UltraPure grade* *CAS 148504-34-1* | 501 | 521 |
Images
Figure 1. Chemical structure for BAPTA, AM *UltraPure Grade* *CAS 126150-97-8*
Citations
View all 9 citations: Citation Explorer
Regulation of Calcium entry by cyclic GMP signaling in Toxoplasma gondii
Authors: Triana, Miryam A Hortua and M{\'a}rquez-Nogueras, Karla M and Fazli, Mojtaba Sedigh and Quinn, Shannon and Moreno, Silvia NJ
Journal: Journal of Biological Chemistry (2024): 105771
Authors: Triana, Miryam A Hortua and M{\'a}rquez-Nogueras, Karla M and Fazli, Mojtaba Sedigh and Quinn, Shannon and Moreno, Silvia NJ
Journal: Journal of Biological Chemistry (2024): 105771
Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
Authors: Yang, Xinhui and Jiang, Jiangtao and Yang, Xinyan and Han, Jichun and Zheng, Qiusheng
Journal: Molecular medicine reports (2016): 911--919
Helicobacter pylori induces IL-1β protein through the inflammasome activation in differentiated macrophagic cells
Authors: Kameoka, Shoichiro and Kameyama, Takeshi and Hayashi, Takaya and Sato, Seiichi and Ohnishi, Naomi and Hayashi, Takeru and Murata-Kamiya, Naoko and Higashi, Hideaki and Hatakeyama, Masanori and Takaoka, Akinori
Journal: Biomedical Research (2016): 21--27
Authors: Kameoka, Shoichiro and Kameyama, Takeshi and Hayashi, Takaya and Sato, Seiichi and Ohnishi, Naomi and Hayashi, Takeru and Murata-Kamiya, Naoko and Higashi, Hideaki and Hatakeyama, Masanori and Takaoka, Akinori
Journal: Biomedical Research (2016): 21--27
Spautin-1 Ameliorates Acute Pancreatitis via Inhibiting Impaired Autophagy and Alleviating Calcium Overload
Authors: Xiao, Juan and Feng, Xueping and Huang, Xiao-Ying and Huang, Zhongshi and Huang, Yanqiang and Li, Chaogan and Li, Genliang and Nong, Song and Wu, Ruoshi and Huang, Yongzhi and others, undefined
Journal: Molecular Medicine (2016): 643
Authors: Xiao, Juan and Feng, Xueping and Huang, Xiao-Ying and Huang, Zhongshi and Huang, Yanqiang and Li, Chaogan and Li, Genliang and Nong, Song and Wu, Ruoshi and Huang, Yongzhi and others, undefined
Journal: Molecular Medicine (2016): 643
Role of Secretory Processes in Cardiac Fibroblasts for Heart Failure Development and Progression
Authors: Kittana, Naim
Journal: (2014)
Authors: Kittana, Naim
Journal: (2014)
Ras guanyl nucleotide releasing protein 2 affects cell viability and cell--matrix adhesion in ECV304 endothelial cells
Authors: Takino, Junichi and Nagamine, Kentaro and Hori, Takamitsu
Journal: Cell adhesion & migration (2013): 262--266
Authors: Takino, Junichi and Nagamine, Kentaro and Hori, Takamitsu
Journal: Cell adhesion & migration (2013): 262--266
Delayed administration of the nucleic acid analog 2Cl-C. OXT-A attenuates brain damage and enhances functional recovery after ischemic stroke
Authors: Okabe, Naohiko and Nakamura, Emi and Himi, Naoyuki and Narita, Kazuhiko and Tsukamoto, Ikuko and Maruyama, Tokumi and Sakakibara, Norikazu and Nakamura, Takehiro and Itano, Toshifumi and Miyamoto, Osamu
Journal: Brain research (2013): 115--131
Authors: Okabe, Naohiko and Nakamura, Emi and Himi, Naoyuki and Narita, Kazuhiko and Tsukamoto, Ikuko and Maruyama, Tokumi and Sakakibara, Norikazu and Nakamura, Takehiro and Itano, Toshifumi and Miyamoto, Osamu
Journal: Brain research (2013): 115--131
Involvement of thioredoxin-binding protein 2 in the antitumor activity of CD437
Authors: Matsuoka, Saori and Tsuchiya, Hiroyuki and Sakabe, Tomohiko and Watanabe, Yumi and Hoshikawa, Yoshiko and Kurimasa, Akihiro and Itamochi, Hiroaki and Harada, Tasuku and Terakawa, Naoki and Masutani, Hiroshi and others, undefined
Journal: Cancer science (2008): 2485--2490
Authors: Matsuoka, Saori and Tsuchiya, Hiroyuki and Sakabe, Tomohiko and Watanabe, Yumi and Hoshikawa, Yoshiko and Kurimasa, Akihiro and Itamochi, Hiroaki and Harada, Tasuku and Terakawa, Naoki and Masutani, Hiroshi and others, undefined
Journal: Cancer science (2008): 2485--2490
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
FAQ
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?