bBBr [Dibromobimane] *CAS 68654-25-1*

bBBr

Dibromobimane; CAS 68654-25-1

dBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. Dibromobimane is a unique fluorogenic crosslinking reagent for proteins because it is unlikely to fluoresce until both of its alkylating groups have reacted. It has been used to crosslink thiols in myosin, hemoglobin, Escherichia coli lactose permease and mitochondrial ATPase. Dibromobimane is also used to probe for the proximity of dual-cysteine mutagenesis sites in ArsA ATPase and to crosslink thiols in actin, myosin and P-glycoprotein.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
634	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of bBBr [Dibromobimane] *CAS 68654-25-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	285.706 µL	1.429 mL	2.857 mL	14.285 mL	28.571 mL
5 mM	57.141 µL	285.706 µL	571.412 µL	2.857 mL	5.714 mL
10 mM	28.571 µL	142.853 µL	285.706 µL	1.429 mL	2.857 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	350.01 g/mol	=	mL	x	mM	=	mmol

References

View all 25 references: Citation Explorer

Reactivity of zinc finger cysteines: chemical modifications within labile zinc fingers in estrogen receptor

Authors:

Atsriku C, Scott GK, Benz CC, Baldwin MA.

Journal:

J Am Soc Mass Spectrom (2005): 2017

Interaction of S100A8/S100A9-arachidonic acid complexes with the scavenger receptor CD36 may facilitate fatty acid uptake by endothelial cells

Authors:

Kerkhoff C, Sorg C, T and on NN, Nacken W.

Journal:

Biochemistry (2001): 241

Determination of plasma homocysteine by high performance liquid chromatography with fluorescence detection

Authors:

Liao Y, Liang YQ, Zhi XM, Liao ZH.

Journal:

Se Pu (2000): 49

Plasma cysteine deficiency and decreased reduction of nitrososulfamethoxazole with HIV infection

Authors:

Naisbitt DJ, Vilar FJ, Stalford AC, Wilkins EG, Pirmohamed M, Park BK.

Journal:

AIDS Res Hum Retroviruses (2000): 1929

Fatty acid translocase/CD36 mediates the uptake of palmitate by type II pneumocytes

Authors:

Guthmann F, Haupt R, Looman AC, Spener F, Rustow B.

Journal:

Am J Physiol (1999): L191

Physical properties

Molecular weight	350.01
Solvent	DMSO

Spectral properties

Excitation (nm)	394
Emission (nm)	490

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	68654-25-1

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Page updated on October 13, 2025