Benzoyl-Arg-AMC

This Benzoyl-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate used for the detection of enzymatic activity of human alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set.

Protocol

SDS

Catalog	Size	Price	Quantity
13478	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	181.984 µL	909.918 µL	1.82 mL	9.099 mL	18.198 mL
5 mM	36.397 µL	181.984 µL	363.967 µL	1.82 mL	3.64 mL
10 mM	18.198 µL	90.992 µL	181.984 µL	909.918 µL	1.82 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	549.5 g/mol	=	mL	x	mM	=	mmol

References

View all 79 references: Citation Explorer

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease

Authors:

Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.

Journal:

Anal Biochem (2009): 195

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model

Authors:

Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.

Journal:

Gynecol Oncol (2009): 616

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7

Authors:

Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.

Journal:

J Biomol Screen (2009): 1

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

Authors:

Nakata H, Ohtsuki T, Sisido M.

Journal:

Anal Biochem (2009): 121

Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes

Authors:

An L, Liu L, Wang S.

Journal:

Biomacromolecules (2009): 454

Physical properties

Molecular weight	549.50
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2025