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Chemical structure for Benzoyl-Arg-AMC
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5 mg 13478 $95

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Ex/Em (nm)339/439
Storage Freeze (<-15 °C)
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Category Enzyme Detection
Peptidases and Proteases
Related Secondary Reagents
This Benzoyl-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate used for the detection of enzymatic activity of human alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has Ex/Em = 351/430 nm, and can be easily detected with the DAPI filter set.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =

Spectrum Advanced Spectrum Viewer

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Wavelength (nm)


A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
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Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
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Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
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Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
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Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
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Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
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[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

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