Benzoyl-Leu-Gly-Arg-Aminoluciferin

This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. Benzoyl-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.

Datasheet

SDS

Catalog	Size	Price	Quantity
13000	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.38 µL	606.899 µL	1.214 mL	6.069 mL	12.138 mL
5 mM	24.276 µL	121.38 µL	242.76 µL	1.214 mL	2.428 mL
10 mM	12.138 µL	60.69 µL	121.38 µL	606.899 µL	1.214 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	823.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant

Authors:

Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio

Journal:

Analytical Biochemistry (2021): 114408

Endotoxin assay by bioluminescence using mutant firefly luciferase

Authors:

Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio

Journal:

Analytical biochemistry (2010): 152--155

References

View all 16 references: Citation Explorer

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins

Authors:

Harwood KR, Mofford DM, Reddy GR, Miller SC.

Journal:

Chem Biol (2011): 1649

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

Authors:

Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.

Journal:

Apoptosis (2011): 198

Aminoluciferins as functional bioluminogenic substrates of firefly luciferase

Authors:

Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.

Journal:

Chem Asian J (2011): 1800

Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin

Authors:

Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.

Journal:

Cell Death Differ (2010): 1003

Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase

Authors:

Reddy, G. R.; Thompson, W. C.; Miller, S. C.

Journal:

J Am Chem Soc (2010): 13586-7

Physical properties

Molecular weight	823.86
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	362
Emission (nm)	499

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 18, 2026