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Benzoyl-Leu-Gly-Arg-Aminoluciferin

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Physical properties
Molecular weight823.86
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)18000
Excitation (nm)362
Emission (nm)499
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


See also: Proteases
Molecular weight
823.86
Extinction coefficient (cm -1 M -1)
18000
Excitation (nm)
362
Emission (nm)
499
This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. Benzoyl-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM121.38 µL606.899 µL1.214 mL6.069 mL12.138 mL
5 mM24.276 µL121.38 µL242.76 µL1.214 mL2.428 mL
10 mM12.138 µL60.69 µL121.38 µL606.899 µL1.214 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)18000
Excitation (nm)362
Emission (nm)499

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
BOC-Leu-Gly-Arg-Aminoluciferin36249918000

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Citations


View all 2 citations: Citation Explorer
Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155

References


View all 16 references: Citation Explorer
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
Homogeneous, bioluminescent proteasome assays
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197