Benzoyl-Leu-Gly-Arg-Aminoluciferin
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 823.86 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Proteases
Molecular weight 823.86 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. Benzoyl-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 121.38 µL | 606.899 µL | 1.214 mL | 6.069 mL | 12.138 mL |
5 mM | 24.276 µL | 121.38 µL | 242.76 µL | 1.214 mL | 2.428 mL |
10 mM | 12.138 µL | 60.69 µL | 121.38 µL | 606.899 µL | 1.214 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
BOC-Leu-Gly-Arg-Aminoluciferin | 362 | 499 | 18000 |
Citations
View all 2 citations: Citation Explorer
Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
References
View all 16 references: Citation Explorer
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
Homogeneous, bioluminescent proteasome assays
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
FAQ
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
What is the best way to administer D-Luciferin potassium salt for in vivo bioluminescent imaging?
Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?
What assay kits measure NADP/NADPH from cell samples?
How should I reconstitute an NADPH standard?
What is the best way to administer D-Luciferin potassium salt for in vivo bioluminescent imaging?
Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?
What assay kits measure NADP/NADPH from cell samples?