Beta-Ala-AMC

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Physical properties

Molecular weight	282.72
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Beta-Ala-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	353.707 µL	1.769 mL	3.537 mL	17.685 mL	35.371 mL
5 mM	70.741 µL	353.707 µL	707.414 µL	3.537 mL	7.074 mL
10 mM	35.371 µL	176.853 µL	353.707 µL	1.769 mL	3.537 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	341
Emission (nm)	441

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
D-Ala-AMC CAS 201847-52-1	341	441	-
Beta-Ala-R110	500	522	80000

Images

Figure 1. Chemical structure for Beta-Ala-AMC.

References

View all 17 references: Citation Explorer

Direct detection of cysteine peptidases for MALDI-TOF MS analysis using fluorogenic substrates.
Authors: Elpidina, Elena N and Semashko, Tatiana A and Smirnova, Yulia A and Dvoryakova, Elena A and Dunaevsky, Yakov E and Belozersky, Mikhail A and Serebryakova, Marina V and Klyachko, Elena V and Abd El-Latif, Ashraf O and Oppert, Brenda and Filippova, Irina Y
Journal: Analytical biochemistry (2019): 45-50

Catalytic Mechanism of Cruzain from Trypanosoma cruzi As Determined from Solvent Kinetic Isotope Effects of Steady-State and Pre-Steady-State Kinetics.
Authors: Zhai, Xiang and Meek, Thomas D
Journal: Biochemistry (2018): 3176-3190

Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani.
Authors: Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A
Journal: International journal of biological macromolecules (2018): 940-954

Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense.
Authors: Pillay, Davita and Boulangé, Alain F V and Coustou, Virginie and Baltz, Théo and Coetzer, Theresa H T
Journal: Experimental parasitology (2013): 675-84

A quantitative technique for determining proteases and their substrate specificities and pH optima in crude enzyme extracts.
Authors: Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz
Journal: Analytical biochemistry (2009): 56-62

Cold-induced apoptosis of rat liver endothelial cells: involvement of the proteasome.
Authors: Doeppner, Thorsten R and Grune, Tilman and de Groot, Herbert and Rauen, Ursula
Journal: Transplantation (2003): 1946-53

Comparison of different peptidase substrates for evaluation of microbial quality of aerobically stored meats.
Authors: Stepaniak, L
Journal: Journal of food protection (2000): 1447-9

[Preparation of novel specific aminopeptidase inhibitors with a cyclic imide skeleton].
Authors: Takahashi, H and Komoda, M and Kakuta, H and Hashimoto, Y
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2000): 909-21

Potent homophthalimide-type inhibitors of B16F10/L5 mouse melanoma cell invasion.
Authors: Kagechika, H and Komoda, M and Fujimoto, Y and Koiso, Y and Takayama, H and Kadoya, S and Miyata, K and Kato, F and Kato, M and Hashimoto, Y
Journal: Biological & pharmaceutical bulletin (1999): 1010-2

Cyclosporin A-cyclophilin complex formation. A model based on X-ray and NMR data.
Authors: Spitzfaden, C and Weber, H P and Braun, W and Kallen, J and Wider, G and Widmer, H and Walkinshaw, M D and Wüthrich, K
Journal: FEBS letters (1992): 291-300