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Beta-Ala-R110

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Physical properties
Molecular weight545.42
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)80000
Excitation (nm)500
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Proteases
Molecular weight
545.42
Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)
500
Emission (nm)
522
Beta-Alanine-R110 is a fluorogenic substrate that might be used for detection pancreatic elastase, arylamidase and beta-alanine aminopeptidase activities. It may also be used for detecting Pseudomonas aeruginosa that have beta-alanine aminopeptidase activity. Beta-alanine aminopeptidase activity was detected in Serratia liquefaciens, Pseudomonas aeruginosa, Pseudomonas fluorescens, Burkholderia cepacia, Pseudomonas mendocina., and Ochrobactrum anthropi. Therefore, beta-Alanine-R110 may be used for detecting these species in food and other samples. Compared to beta-Alanine-AMC (#13202), beta-Alanine-R110 is more sensitive. Its enzymatic product can be readily detected with the common FITC channel or FITC filter set.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Beta-Ala-R110 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM183.345 µL916.725 µL1.833 mL9.167 mL18.334 mL
5 mM36.669 µL183.345 µL366.69 µL1.833 mL3.667 mL
10 mM18.334 µL91.672 µL183.345 µL916.725 µL1.833 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)80000
Excitation (nm)500
Emission (nm)522

Product Family


NameExcitation (nm)Emission (nm)
Beta-Ala-AMC341441

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References


View all 17 references: Citation Explorer
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Authors: Elpidina, Elena N and Semashko, Tatiana A and Smirnova, Yulia A and Dvoryakova, Elena A and Dunaevsky, Yakov E and Belozersky, Mikhail A and Serebryakova, Marina V and Klyachko, Elena V and Abd El-Latif, Ashraf O and Oppert, Brenda and Filippova, Irina Y
Journal: Analytical biochemistry (2019): 45-50
Catalytic Mechanism of Cruzain from Trypanosoma cruzi As Determined from Solvent Kinetic Isotope Effects of Steady-State and Pre-Steady-State Kinetics.
Authors: Zhai, Xiang and Meek, Thomas D
Journal: Biochemistry (2018): 3176-3190
Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani.
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Journal: International journal of biological macromolecules (2018): 940-954
Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense.
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Journal: Experimental parasitology (2013): 675-84
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Comparison of different peptidase substrates for evaluation of microbial quality of aerobically stored meats.
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Journal: Journal of food protection (2000): 1447-9
[Preparation of novel specific aminopeptidase inhibitors with a cyclic imide skeleton].
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Potent homophthalimide-type inhibitors of B16F10/L5 mouse melanoma cell invasion.
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Journal: Biological & pharmaceutical bulletin (1999): 1010-2
Cyclosporin A-cyclophilin complex formation. A model based on X-ray and NMR data.
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