Beta-Ala-R110
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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International | See distributors |
Bulk request | Inquire |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 545.42 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Proteases
Molecular weight 545.42 | Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 500 | Emission (nm) 522 |
Beta-Alanine-R110 is a fluorogenic substrate that might be used for detection pancreatic elastase, arylamidase and beta-alanine aminopeptidase activities. It may also be used for detecting Pseudomonas aeruginosa that have beta-alanine aminopeptidase activity. Beta-alanine aminopeptidase activity was detected in Serratia liquefaciens, Pseudomonas aeruginosa, Pseudomonas fluorescens, Burkholderia cepacia, Pseudomonas mendocina., and Ochrobactrum anthropi. Therefore, beta-Alanine-R110 may be used for detecting these species in food and other samples. Compared to beta-Alanine-AMC (#13202), beta-Alanine-R110 is more sensitive. Its enzymatic product can be readily detected with the common FITC channel or FITC filter set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Beta-Ala-R110 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 183.345 µL | 916.725 µL | 1.833 mL | 9.167 mL | 18.334 mL |
5 mM | 36.669 µL | 183.345 µL | 366.69 µL | 1.833 mL | 3.667 mL |
10 mM | 18.334 µL | 91.672 µL | 183.345 µL | 916.725 µL | 1.833 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
References
View all 17 references: Citation Explorer
Direct detection of cysteine peptidases for MALDI-TOF MS analysis using fluorogenic substrates.
Authors: Elpidina, Elena N and Semashko, Tatiana A and Smirnova, Yulia A and Dvoryakova, Elena A and Dunaevsky, Yakov E and Belozersky, Mikhail A and Serebryakova, Marina V and Klyachko, Elena V and Abd El-Latif, Ashraf O and Oppert, Brenda and Filippova, Irina Y
Journal: Analytical biochemistry (2019): 45-50
Authors: Elpidina, Elena N and Semashko, Tatiana A and Smirnova, Yulia A and Dvoryakova, Elena A and Dunaevsky, Yakov E and Belozersky, Mikhail A and Serebryakova, Marina V and Klyachko, Elena V and Abd El-Latif, Ashraf O and Oppert, Brenda and Filippova, Irina Y
Journal: Analytical biochemistry (2019): 45-50
Catalytic Mechanism of Cruzain from Trypanosoma cruzi As Determined from Solvent Kinetic Isotope Effects of Steady-State and Pre-Steady-State Kinetics.
Authors: Zhai, Xiang and Meek, Thomas D
Journal: Biochemistry (2018): 3176-3190
Authors: Zhai, Xiang and Meek, Thomas D
Journal: Biochemistry (2018): 3176-3190
Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani.
Authors: Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A
Journal: International journal of biological macromolecules (2018): 940-954
Authors: Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A
Journal: International journal of biological macromolecules (2018): 940-954
Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense.
Authors: Pillay, Davita and Boulangé, Alain F V and Coustou, Virginie and Baltz, Théo and Coetzer, Theresa H T
Journal: Experimental parasitology (2013): 675-84
Authors: Pillay, Davita and Boulangé, Alain F V and Coustou, Virginie and Baltz, Théo and Coetzer, Theresa H T
Journal: Experimental parasitology (2013): 675-84
A quantitative technique for determining proteases and their substrate specificities and pH optima in crude enzyme extracts.
Authors: Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz
Journal: Analytical biochemistry (2009): 56-62
Authors: Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz
Journal: Analytical biochemistry (2009): 56-62
Cold-induced apoptosis of rat liver endothelial cells: involvement of the proteasome.
Authors: Doeppner, Thorsten R and Grune, Tilman and de Groot, Herbert and Rauen, Ursula
Journal: Transplantation (2003): 1946-53
Authors: Doeppner, Thorsten R and Grune, Tilman and de Groot, Herbert and Rauen, Ursula
Journal: Transplantation (2003): 1946-53
Comparison of different peptidase substrates for evaluation of microbial quality of aerobically stored meats.
Authors: Stepaniak, L
Journal: Journal of food protection (2000): 1447-9
Authors: Stepaniak, L
Journal: Journal of food protection (2000): 1447-9
[Preparation of novel specific aminopeptidase inhibitors with a cyclic imide skeleton].
Authors: Takahashi, H and Komoda, M and Kakuta, H and Hashimoto, Y
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2000): 909-21
Authors: Takahashi, H and Komoda, M and Kakuta, H and Hashimoto, Y
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2000): 909-21
Potent homophthalimide-type inhibitors of B16F10/L5 mouse melanoma cell invasion.
Authors: Kagechika, H and Komoda, M and Fujimoto, Y and Koiso, Y and Takayama, H and Kadoya, S and Miyata, K and Kato, F and Kato, M and Hashimoto, Y
Journal: Biological & pharmaceutical bulletin (1999): 1010-2
Authors: Kagechika, H and Komoda, M and Fujimoto, Y and Koiso, Y and Takayama, H and Kadoya, S and Miyata, K and Kato, F and Kato, M and Hashimoto, Y
Journal: Biological & pharmaceutical bulletin (1999): 1010-2
Cyclosporin A-cyclophilin complex formation. A model based on X-ray and NMR data.
Authors: Spitzfaden, C and Weber, H P and Braun, W and Kallen, J and Wider, G and Widmer, H and Walkinshaw, M D and Wüthrich, K
Journal: FEBS letters (1992): 291-300
Authors: Spitzfaden, C and Weber, H P and Braun, W and Kallen, J and Wider, G and Widmer, H and Walkinshaw, M D and Wüthrich, K
Journal: FEBS letters (1992): 291-300
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Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
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FAQ
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What is the best way to administer D-Luciferin potassium salt for in vivo bioluminescent imaging?
Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?
What assay kits measure NADP/NADPH from cell samples?