Beta-Ala-R110-ML

Fluorometric methods based on the peptide substrates labeled with cleavable fluorophores have been widely used for assaying various proteases. The two dominant classes are AMC-based (coumarin dye) and R110-based (rhodamine 110) substrates. AMC substrates are much less sensitive due to their short wavelength, low extinction coefficient and high fluorescent background resulted from the autofluorescence of biological samples. R110-based peptide substrates have a longer excitation and emission wavelength, low background signal, and being highly fluorescent. However, R110-based peptide substrates carries two peptide blocking groups that need to be cleaved from their bis-peptide substrates in order to generate maximal signal. This two-step cleavage severely limits the linear dynamic range of R110-based peptide substrates. N-morpholinecarbonyl-R110 (R110-ML) has been successfully used to overcome both the poor cell penetration and the kinetic limitation of R110-based peptide substrates. R110-ML substrates are as sensitive as R110 substrates for most of protease detections. Beta-Ala-R110-ML is a fluorogenic substrate that might be used for detecting aminopeptidase M substrate, alanyl aminopeptidase and trypsin activities. Beta-Ala-R110-ML may be used for detecting some bacteria in food and other samples by monitoring their alanyl aminopeptidase activities. Compared to Ala-AMC (#13458), Beta-Ala-R110-ML is more sensitive. Its enzymatic product can be readily detected with the common FITC channel or FITC filter set.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13205	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Beta-Ala-R110-ML to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.094 µL	795.469 µL	1.591 mL	7.955 mL	15.909 mL
5 mM	31.819 µL	159.094 µL	318.188 µL	1.591 mL	3.182 mL
10 mM	15.909 µL	79.547 µL	159.094 µL	795.469 µL	1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	628.56 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Design, synthesis and biological evaluation of substituted 3-amino-N-(thiazol-2-yl)pyrazine-2-carboxamides as inhibitors of mycobacterial methionine aminopeptidase 1.

Authors:

Juhás, Martin and Pallabothula, Vinod S K and Grabrijan, Katarina and Šimovičová, Martina and Janďourek, Ondřej and Konečná, Klára and Bárta, Pavel and Paterová, Pavla and Gobec, Stanislav and Sosič, Izidor and Zitko, Jan

Journal:

Bioorganic chemistry (2022): 105489

A Potent Inhibitor of Aminopeptidase P2 Reduces Reperfusion Injury in Models of Myocardial Infarction and Stroke.

Authors:

Lenz, Morgan Rae and Tsai, Shih-Yen and Roessler, Anne E and Wang, Yang and Sethupathi, Periannan and Jones, Walter Keith and Kartje, Gwendolyn L and Simmons, William Howard

Journal:

The Journal of pharmacology and experimental therapeutics (2022)

The dual-targeted prolyl aminopeptidase PAP1 is involved in proline accumulation in response to stress and during pollen development.

Authors:

Ghifari, Abi S and Teixeira, Pedro F and Kmiec, Beata and Singh, Neha and Glaser, Elzbieta and Murcha, Monika W

Journal:

Journal of experimental botany (2022): 78-93

Characterization and heterologous expression of a novel Co2+-dependent leucyl aminopeptidase Amp0279 originating from Lysinibacillus sphaericus.

Authors:

Zhao, Puying and Zhang, Meng and Wan, Xiaofu and Geng, Peiling and Xiong, Hairong and Hu, Xiaomin

Journal:

Applied microbiology and biotechnology (2022)

Discovery of Selective Inhibitor Leads by Targeting an Allosteric Site in Insulin-Regulated Aminopeptidase.

Authors:

Temponeras, Ioannis and Chiniadis, Lykourgos and Papakyriakou, Athanasios and Stratikos, Efstratios

Journal:

Pharmaceuticals (Basel, Switzerland) (2021)

Physical properties

Molecular weight	628.56
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 21, 2025