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Beta-trifluoromethylumbelliferone [7-Hydroxy-4-trifluoromethylcoumarin] *CAS 575-03-1*

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Physical properties
Molecular weight230.14
Spectral properties
Excitation (nm)385
Emission (nm)499
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
Excitation (nm)
Emission (nm)
This coumarin compound is used as a calibration standard for 7-hydroxy-4-trifluoromethylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Beta-trifluoromethylumbelliferone [7-Hydroxy-4-trifluoromethylcoumarin] *CAS 575-03-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM434.518 µL2.173 mL4.345 mL21.726 mL43.452 mL
5 mM86.904 µL434.518 µL869.036 µL4.345 mL8.69 mL
10 mM43.452 µL217.259 µL434.518 µL2.173 mL4.345 mL

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Spectral properties

Excitation (nm)385
Emission (nm)499



View all 1 citations: Citation Explorer
Reduced biotransformation of polycyclic aromatic hydrocarbons (PAHs) in pollution-adapted Gulf killifish (Fundulus grandis)
Authors: Franco, Marco E and Johanning, Karla and Matson, Cole W and Lavado, Ramon
Journal: Science of The Total Environment (2021): 150854


View all 15 references: Citation Explorer
Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomes
Authors: Chang TK, Crespi CL, Waxman DJ.
Journal: Methods Mol Biol (2006): 97
Identification of human UDP-glucuronosyltransferase enzyme(s) responsible for the glucuronidation of posaconazole (Noxafil)
Authors: Ghosal A, Hapangama N, Yuan Y, Achanfuo-Yeboah J, Iannucci R, Chowdhury S, Alton K, Patrick JE, Zbaida S.
Journal: Drug Metab Dispos (2004): 267
Silybin inactivates cytochromes P450 3A4 and 2C9 and inhibits major hepatic glucuronosyltransferases
Authors: Sridar C, Goosen TC, Kent UM, Williams JA, Hollenberg PF.
Journal: Drug Metab Dispos (2004): 587
Functional characterization of wild-type and variant (T202I and M59I) human UDP-glucuronosyltransferase 1A10
Authors: Jinno H, Saeki M, Tanaka-Kagawa T, Hanioka N, Saito Y, Ozawa S, Ando M, Shirao K, Minami H, Ohtsu A, Yoshida T, Saijo N, Sawada J.
Journal: Drug Metab Dispos (2003): 528
Generation and validation of rapid computational filters for cyp2d6 and cyp3a4
Authors: Ekins S, Berbaum J, Harrison RK.
Journal: Drug Metab Dispos (2003): 1077
Effects of plant-derived phenols on rat liver cytochrome P450 2B1 activity
Authors: Huynh HT, Teel RW.
Journal: Anticancer Res (2002): 1699
Metabolism of 2,5-bis(trifluoromethyl)-7-benzyloxy-4-trifluoromethylcoumarin by human hepatic CYP isoforms: evidence for selectivity towards CYP3A4
Authors: Renwick AB, Lewis DF, Fulford S, Surry D, Williams B, Worboys PD, Cai X, Wang RW, Price RJ, Lake BG, Evans DC.
Journal: Xenobiotica (2001): 187
Evaluation of 7-benzyloxy-4-trifluoromethylcoumarin, some other 7-hydroxy-4-trifluoromethylcoumarin derivatives and 7-benzyloxyquinoline as fluorescent substrates for rat hepatic cytochrome P450 enzymes
Authors: Renwick AB, Lavignette G, Worboy PD, Williams B, Surry D, Lewis DF, Price RJ, Lake BG, Evans DC.
Journal: Xenobiotica (2001): 861
Metabolism of 7-benzyloxy-4-trifluoromethyl-coumarin by human hepatic cytochrome P450 isoforms
Authors: Renwick AB, Surry D, Price RJ, Lake BG, Evans DC.
Journal: Xenobiotica (2000): 955
CYP isoform induction screening in 96-well plates: use of 7-benzyloxy-4-trifluoromethylcoumarin as a substrate for studies with rat hepatocytes
Authors: Price RJ, Surry D, Renwick AB, Meneses-Lorente G, Lake BG, Evans DC.
Journal: Xenobiotica (2000): 781