Calculator
BG-Maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 421.42 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| BG Succinimidyl Ester |
| Overview |  SDSProtocol |
Molecular weight 421.42 |
SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. BG Maleimide is a thiol-reactive building block for preparing of SNAP substrates from thiol-containing labels or biological targets. BG Maleimide building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BG-Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 237.293 µL | 1.186 mL | 2.373 mL | 11.865 mL | 23.729 mL |
| 5 mM | 47.459 µL | 237.293 µL | 474.586 µL | 2.373 mL | 4.746 mL |
| 10 mM | 23.729 µL | 118.646 µL | 237.293 µL | 1.186 mL | 2.373 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Chemical structure for BG-Malaimide.
References
View all 12 references: Citation Explorer
Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
Fluorescent Probe Encapsulated in SNAP-Tag Protein Cavity To Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity.
Authors: Zeng, Yan-Syun and Gao, Ruo-Cing and Wu, Ting-Wei and Cho, Chien and Tan, Kui-Thong
Journal: Bioconjugate chemistry (2016): 1872-9
Authors: Zeng, Yan-Syun and Gao, Ruo-Cing and Wu, Ting-Wei and Cho, Chien and Tan, Kui-Thong
Journal: Bioconjugate chemistry (2016): 1872-9
Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein-Protein Complexes in Living Cells.
Authors: Mie, Masayasu and Naoki, Tatsuhiko and Kobatake, Eiry
Journal: Analytical chemistry (2016): 8166-71
Authors: Mie, Masayasu and Naoki, Tatsuhiko and Kobatake, Eiry
Journal: Analytical chemistry (2016): 8166-71
Visualization of phagosomal hydrogen peroxide production by a novel fluorescent probe that is localized via SNAP-tag labeling.
Authors: Abo, Masahiro and Minakami, Reiko and Miyano, Kei and Kamiya, Mako and Nagano, Tetsuo and Urano, Yasuteru and Sumimoto, Hideki
Journal: Analytical chemistry (2014): 5983-90
Authors: Abo, Masahiro and Minakami, Reiko and Miyano, Kei and Kamiya, Mako and Nagano, Tetsuo and Urano, Yasuteru and Sumimoto, Hideki
Journal: Analytical chemistry (2014): 5983-90
Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor.
Authors: Shih, Po-Ming and Liu, Tao-Kai and Tan, Kui-Thong
Journal: Chemical communications (Cambridge, England) (2013): 6212-4
Authors: Shih, Po-Ming and Liu, Tao-Kai and Tan, Kui-Thong
Journal: Chemical communications (Cambridge, England) (2013): 6212-4
Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells.
Authors: Mie, Masayasu and Naoki, Tatsuhiko and Uchida, Kentaro and Kobatake, Eiry
Journal: The Analyst (2012): 4760-5
Authors: Mie, Masayasu and Naoki, Tatsuhiko and Uchida, Kentaro and Kobatake, Eiry
Journal: The Analyst (2012): 4760-5
Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging.
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and Xu, Ming-Qun and Corrêa, Ivan R
Journal: Chembiochem : a European journal of chemical biology (2011): 2217-26
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and Xu, Ming-Qun and Corrêa, Ivan R
Journal: Chembiochem : a European journal of chemical biology (2011): 2217-26
One- and two-photon live cell imaging using a mutant SNAP-Tag protein and its FRET substrate pairs.
Authors: Zhang, Chong-Jing and Li, Lin and Chen, Grace Y J and Xu, Qing-Hua and Yao, Shao Q
Journal: Organic letters (2011): 4160-3
Authors: Zhang, Chong-Jing and Li, Lin and Chen, Grace Y J and Xu, Qing-Hua and Yao, Shao Q
Journal: Organic letters (2011): 4160-3
Organelle-targetable fluorescent probes for imaging hydrogen peroxide in living cells via SNAP-Tag protein labeling.
Authors: Srikun, Duangkhae and Albers, Aaron E and Nam, Christine I and Iavarone, Anthony T and Chang, Christopher J
Journal: Journal of the American Chemical Society (2010): 4455-65
Authors: Srikun, Duangkhae and Albers, Aaron E and Nam, Christine I and Iavarone, Anthony T and Chang, Christopher J
Journal: Journal of the American Chemical Society (2010): 4455-65