BG-Maleimide

SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. BG Maleimide is a thiol-reactive building block for preparing of SNAP substrates from thiol-containing labels or biological targets. BG Maleimide building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12566	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BG-Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	237.293 µL	1.186 mL	2.373 mL	11.865 mL	23.729 mL
5 mM	47.459 µL	237.293 µL	474.586 µL	2.373 mL	4.746 mL
10 mM	23.729 µL	118.646 µL	237.293 µL	1.186 mL	2.373 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	421.42 g/mol	=	mL	x	mM	=	mmol

References

View all 12 references: Citation Explorer

Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.

Authors:

Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing

Journal:

Chemical communications (Cambridge, England) (2020): 6074-6077

Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.

Authors:

Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi

Journal:

Biosensors & bioelectronics (2017): 313-320

Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein-Protein Complexes in Living Cells.

Authors:

Mie, Masayasu and Naoki, Tatsuhiko and Kobatake, Eiry

Journal:

Analytical chemistry (2016): 8166-71

Fluorescent Probe Encapsulated in SNAP-Tag Protein Cavity To Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity.

Authors:

Zeng, Yan-Syun and Gao, Ruo-Cing and Wu, Ting-Wei and Cho, Chien and Tan, Kui-Thong

Journal:

Bioconjugate chemistry (2016): 1872-9

Visualization of phagosomal hydrogen peroxide production by a novel fluorescent probe that is localized via SNAP-tag labeling.

Authors:

Abo, Masahiro and Minakami, Reiko and Miyano, Kei and Kamiya, Mako and Nagano, Tetsuo and Urano, Yasuteru and Sumimoto, Hideki

Journal:

Analytical chemistry (2014): 5983-90

Physical properties

Molecular weight	421.42
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 23, 2025