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BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 270.30 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
CAS 674799-96-3 | Molecular weight 270.30 |
BG-NH2 is a carboxy-reactive building block for preparing of SNAP substrates from the NHS esters or other amine-reactive forms of labels or biological targets. This BG-NH2 building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications. AAT Bioquest offers a broad range of amine-reactive fluorescent probes and other Tag probes that can be readily coupled to BG-NH2. The SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 369.959 µL | 1.85 mL | 3.7 mL | 18.498 mL | 36.996 mL |
| 5 mM | 73.992 µL | 369.959 µL | 739.919 µL | 3.7 mL | 7.399 mL |
| 10 mM | 36.996 µL | 184.98 µL | 369.959 µL | 1.85 mL | 3.7 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for BG-NH2 [O6-(4-Aminomethyl-benzyl)guanine].