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BG-SiR Conjugate
Site-specific labeling of cellular proteins with chemical probes is a powerful tool for studying protein functions in living cells. The SNAP-tag is a modified form of the DNA repair enzyme, human O6-alkylguanine-DNA-alkyltransferase (AGT) and undergoes a self-labeling reaction to form a covalent bond with O6-benzylguanine (BG) derivatives. BG-SiR conjugate is a fluorescent SNAP substrate that can be used to label SNAP tag-fused target proteins. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. SNAP-SiR substrate enables the specific, covalent attachment of SiR fluorophore to a protein of interest. SiR has a similar fluorescence spectrum to Cy5 or Alexa Fluor 647. The labeled proteins can be imaged with Cy5 filter set.
References
View all 15 references: Citation Explorer
Hydrogen Bond-Enhanced Nanoaggregation and Antisolvatochromic Fluorescence for Protein-Recognition by Si-Coumarins.
Authors: Li, Chen and Wang, Ting and Fan, Mengting and Wang, Ning and Lin, Xiaofeng and Sun, Yan and Cui, Xiaoyan
Journal: Nano letters (2022): 1954-1962
Authors: Li, Chen and Wang, Ting and Fan, Mengting and Wang, Ning and Lin, Xiaofeng and Sun, Yan and Cui, Xiaoyan
Journal: Nano letters (2022): 1954-1962
Magnetic nanoparticles covalently immobilizing epidermal growth factor receptor by SNAP-Tag protein as a platform for drug discovery.
Authors: Zhou, Huaxin and Fu, Jia and Jia, Qianqian and Wang, Saisai and Liang, Peida and Wang, Yamin and Lv, Yanni and Han, Shengli
Journal: Talanta (2022): 123204
Authors: Zhou, Huaxin and Fu, Jia and Jia, Qianqian and Wang, Saisai and Liang, Peida and Wang, Yamin and Lv, Yanni and Han, Shengli
Journal: Talanta (2022): 123204
Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.
Authors: Vettone, Antonella and Serpe, Mario and Hidalgo, Aurelio and Berenguer, José and del Monaco, Giovanni and Valenti, Anna and Rossi, Mosé and Ciaramella, Maria and Perugino, Giuseppe
Journal: Extremophiles : life under extreme conditions (2016): 1-13
Authors: Vettone, Antonella and Serpe, Mario and Hidalgo, Aurelio and Berenguer, José and del Monaco, Giovanni and Valenti, Anna and Rossi, Mosé and Ciaramella, Maria and Perugino, Giuseppe
Journal: Extremophiles : life under extreme conditions (2016): 1-13
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