Biocytin C2 maleimide

Biocytin maleimide readily reacts with thiol moieties of biopolymers to form thioether conjugate that is quite stable. This biocytin maleimide requires mild conjugation conditions. For example, pH of 5.5-8.5 is usually optimal for modifying cysteine residues, and air exposure of reaction solution should be minimized whenever possible to avoid the air oxidation of thiol substrates. Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction. Reactions with this biotinylation reagent should be performed in buffers free of extraneous thiols (such as 6-mercaptoethanol, dithiothreitol and mercaptoethylamine). Proteins or peptides to be biotinylated by thiol-reactive reagents must have a free thiol group (SH) available.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3085	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biocytin C2 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	164.303 µL	821.517 µL	1.643 mL	8.215 mL	16.43 mL
5 mM	32.861 µL	164.303 µL	328.607 µL	1.643 mL	3.286 mL
10 mM	16.43 µL	82.152 µL	164.303 µL	821.517 µL	1.643 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	608.63 g/mol	=	mL	x	mM	=	mmol

References

View all 51 references: Citation Explorer

The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts

Authors:

Xue HG, Yang CY, Ito H.

Journal:

Brain Res Brain Res Protoc (2004): 106

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure

Authors:

Morozov Y, Khalilov I, Ben-Ari Y, Represa A.

Journal:

J Neurosci Methods (2002): 81

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells

Authors:

Baur B, Suormala T, Baumgartner ER.

Journal:

Brain Res (2002): 111

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan

Authors:

Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.

Journal:

Clin Chim Acta (2001): 71

Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier

Authors:

Baur B, Baumgartner ER.

Journal:

Brain Res (2000): 348

Physical properties

Molecular weight	608.63
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200
CAS	1248344-33-3

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Page updated on October 13, 2025