Biocytin *CAS 576-19-2*

Biocytin

CAS 576-19-2

Biocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile marker for neuroanatomical investigations. Biocytin may be injected into brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels. It is taken up by neurons and rapidly transported down to axons in an anterograde fashion. Biocytin can also be used in retrograde tract tracing experiments, although in some cases it appears that fibers must be damaged to produce such labeling.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3080	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biocytin *CAS 576-19-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	268.471 µL	1.342 mL	2.685 mL	13.424 mL	26.847 mL
5 mM	53.694 µL	268.471 µL	536.942 µL	2.685 mL	5.369 mL
10 mM	26.847 µL	134.235 µL	268.471 µL	1.342 mL	2.685 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	372.48 g/mol	=	mL	x	mM	=	mmol

References

View all 51 references: Citation Explorer

The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts

Authors:

Xue HG, Yang CY, Ito H.

Journal:

Brain Res Brain Res Protoc (2004): 106

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure

Authors:

Morozov Y, Khalilov I, Ben-Ari Y, Represa A.

Journal:

J Neurosci Methods (2002): 81

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells

Authors:

Baur B, Suormala T, Baumgartner ER.

Journal:

Brain Res (2002): 111

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan

Authors:

Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.

Journal:

Clin Chim Acta (2001): 71

Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier

Authors:

Baur B, Baumgartner ER.

Journal:

Brain Res (2000): 348

Physical properties

Molecular weight	372.48
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	576-19-2

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