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Biocytin *CAS 576-19-2*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 372.48 |
| Solvent | Water |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Neurodegeneration & Amyloid Staining
CAS 576-19-2 | Molecular weight 372.48 |
Biocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile marker for neuroanatomical investigations. Biocytin may be injected into brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels. It is taken up by neurons and rapidly transported down to axons in an anterograde fashion. Biocytin can also be used in retrograde tract tracing experiments, although in some cases it appears that fibers must be damaged to produce such labeling.
Example protocol
AT A GLANCE
Important notes
It is important to store at 2 to 8°C and should be stored in cool, dark place.
It can be used within 3 years from the date of receipt.
PREPARATION OF WORKING SOLUTION
Biocytin working solution:
Add water to make Biocytin working solution.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Biocytin *CAS 576-19-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 268.471 µL | 1.342 mL | 2.685 mL | 13.424 mL | 26.847 mL |
| 5 mM | 53.694 µL | 268.471 µL | 536.942 µL | 2.685 mL | 5.369 mL |
| 10 mM | 26.847 µL | 134.235 µL | 268.471 µL | 1.342 mL | 2.685 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for Biocytin *CAS 576-19-2*
References
View all 51 references: Citation Explorer
The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106
Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81
Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111
A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71
Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348
In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1
A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189
A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263
A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963
Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167