Biocytin hydrazide CAS 102743-85-1

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Physical properties

Molecular weight	386.51
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biocytin hydrazide *CAS 102743-85-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	258.726 µL	1.294 mL	2.587 mL	12.936 mL	25.873 mL
5 mM	51.745 µL	258.726 µL	517.451 µL	2.587 mL	5.175 mL
10 mM	25.873 µL	129.363 µL	258.726 µL	1.294 mL	2.587 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
ICG hydrazide	789	813	230000	0.076

Images

Figure 1. Chemical structure for Biocytin hydrazide *CAS 102743-85-1*

Citations

View all 2 citations: Citation Explorer

Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms
Authors: Klingler, Florian and Mathias, Sven and Schneider, Helga and Buck, Theresa and Raab, Nadja and Zeh, Nikolas and Shieh, Yu-Wei and Pfannstiel, Jens and Otte, Kerstin
Journal: Biotechnology and Bioengineering (2021)

A short tandem repeat-enriched RNA assembles a nuclear compartment to control alternative splicing and promote cell survival
Authors: Yap, Karen and Mukhina, Svetlana and Zhang, Gen and Tan, Jason SC and Ong, Hong Sheng and Makeyev, Eugene V
Journal: Molecular cell (2018)

References

View all 51 references: Citation Explorer

The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71

Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348

In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1

A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189

A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263

A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963

Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167