Biocytin hydrazide *CAS 102743-85-1*

Biocytin hydrazide

CAS 102743-85-1

This water-soluble biocytin hydrazide is used for the selective non-radioactive detection of glycoconjugates. The method involves either chemical (periodate-induced) or enzymatic (via galactose oxidase) oxidation of glycoconjugates, the resultant aldehyde groups are then labeled with biocytin hydrazide, followed by interaction with an avidin-based enzyme probe.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3086	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biocytin hydrazide *CAS 102743-85-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	258.726 µL	1.294 mL	2.587 mL	12.936 mL	25.873 mL
5 mM	51.745 µL	258.726 µL	517.451 µL	2.587 mL	5.175 mL
10 mM	25.873 µL	129.363 µL	258.726 µL	1.294 mL	2.587 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	386.51 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms

Authors:

Klingler, Florian and Mathias, Sven and Schneider, Helga and Buck, Theresa and Raab, Nadja and Zeh, Nikolas and Shieh, Yu-Wei and Pfannstiel, Jens and Otte, Kerstin

Journal:

Biotechnology and Bioengineering (2021)

A short tandem repeat-enriched RNA assembles a nuclear compartment to control alternative splicing and promote cell survival

Authors:

Yap, Karen and Mukhina, Svetlana and Zhang, Gen and Tan, Jason SC and Ong, Hong Sheng and Makeyev, Eugene V

Journal:

Molecular cell (2018)

References

View all 51 references: Citation Explorer

The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts

Authors:

Xue HG, Yang CY, Ito H.

Journal:

Brain Res Brain Res Protoc (2004): 106

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure

Authors:

Morozov Y, Khalilov I, Ben-Ari Y, Represa A.

Journal:

J Neurosci Methods (2002): 81

Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells

Authors:

Baur B, Suormala T, Baumgartner ER.

Journal:

Brain Res (2002): 111

A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan

Authors:

Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.

Journal:

Clin Chim Acta (2001): 71

Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier

Authors:

Baur B, Baumgartner ER.

Journal:

Brain Res (2000): 348

Physical properties

Molecular weight	386.51
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200
CAS	102743-85-1

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Page updated on October 13, 2025