Biocytin hydrazide *CAS 102743-85-1*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 386.51 |
| Solvent | Water |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C) |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: RNA Purification & Analysis
CAS 102743-85-1 | Molecular weight 386.51 |
This water-soluble biocytin hydrazide is used for the selective non-radioactive detection of glycoconjugates. The method involves either chemical (periodate-induced) or enzymatic (via galactose oxidase) oxidation of glycoconjugates, the resultant aldehyde groups are then labeled with biocytin hydrazide, followed by interaction with an avidin-based enzyme probe.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Biocytin hydrazide *CAS 102743-85-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 258.726 µL | 1.294 mL | 2.587 mL | 12.936 mL | 25.873 mL |
| 5 mM | 51.745 µL | 258.726 µL | 517.451 µL | 2.587 mL | 5.175 mL |
| 10 mM | 25.873 µL | 129.363 µL | 258.726 µL | 1.294 mL | 2.587 mL |
Molarity calculator
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Images
Figure 1. Chemical structure for Biocytin hydrazide *CAS 102743-85-1*
Citations
View all 2 citations: Citation Explorer
Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms
Authors: Klingler, Florian and Mathias, Sven and Schneider, Helga and Buck, Theresa and Raab, Nadja and Zeh, Nikolas and Shieh, Yu-Wei and Pfannstiel, Jens and Otte, Kerstin
Journal: Biotechnology and Bioengineering (2021)
Authors: Klingler, Florian and Mathias, Sven and Schneider, Helga and Buck, Theresa and Raab, Nadja and Zeh, Nikolas and Shieh, Yu-Wei and Pfannstiel, Jens and Otte, Kerstin
Journal: Biotechnology and Bioengineering (2021)
A short tandem repeat-enriched RNA assembles a nuclear compartment to control alternative splicing and promote cell survival
Authors: Yap, Karen and Mukhina, Svetlana and Zhang, Gen and Tan, Jason SC and Ong, Hong Sheng and Makeyev, Eugene V
Journal: Molecular cell (2018)
Authors: Yap, Karen and Mukhina, Svetlana and Zhang, Gen and Tan, Jason SC and Ong, Hong Sheng and Makeyev, Eugene V
Journal: Molecular cell (2018)
References
View all 51 references: Citation Explorer
The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106
Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81
Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111
A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71
Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348
In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1
A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189
A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263
A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963
Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167
Authors: Scalia F, Galoyan SM, Eisner S, Harris E, Su W.
Journal: J Neurosci Methods (1997): 167