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Biocytin hydrazide *CAS 102743-85-1*

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Physical properties
Molecular weight386.51
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)


Molecular weight
This water-soluble biocytin hydrazide is used for the selective non-radioactive detection of glycoconjugates. The method involves either chemical (periodate-induced) or enzymatic (via galactose oxidase) oxidation of glycoconjugates, the resultant aldehyde groups are then labeled with biocytin hydrazide, followed by interaction with an avidin-based enzyme probe.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biocytin hydrazide *CAS 102743-85-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM258.726 µL1.294 mL2.587 mL12.936 mL25.873 mL
5 mM51.745 µL258.726 µL517.451 µL2.587 mL5.175 mL
10 mM25.873 µL129.363 µL258.726 µL1.294 mL2.587 mL

Molarity calculator

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Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
ICG hydrazide7898132300000.076



View all 2 citations: Citation Explorer
Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms
Authors: Klingler, Florian and Mathias, Sven and Schneider, Helga and Buck, Theresa and Raab, Nadja and Zeh, Nikolas and Shieh, Yu-Wei and Pfannstiel, Jens and Otte, Kerstin
Journal: Biotechnology and Bioengineering (2021)
A short tandem repeat-enriched RNA assembles a nuclear compartment to control alternative splicing and promote cell survival
Authors: Yap, Karen and Mukhina, Svetlana and Zhang, Gen and Tan, Jason SC and Ong, Hong Sheng and Makeyev, Eugene V
Journal: Molecular cell (2018)


View all 51 references: Citation Explorer
The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts
Authors: Xue HG, Yang CY, Ito H.
Journal: Brain Res Brain Res Protoc (2004): 106
Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure
Authors: Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
Journal: J Neurosci Methods (2002): 81
Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells
Authors: Baur B, Suormala T, Baumgartner ER.
Journal: Brain Res (2002): 111
A new quantitative analytical method of serum biotinidase activity using biocytin as a substrate and its clinical significance in Japan
Authors: Kumasaka K, Muratsugu M, Fukui T, Kimura M, Takagi Y, Hashizume N.
Journal: Clin Chim Acta (2001): 71
Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier
Authors: Baur B, Baumgartner ER.
Journal: Brain Res (2000): 348
In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing tissue
Authors: Chang SL, LoTurco JJ, Nisenbaum LK.
Journal: J Neurosci Methods (2000): 1
A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue
Authors: Erisir A, Aoki C.
Journal: J Neurosci Methods (1998): 189
A rapid method for combined laser scanning confocal microscopic and electron microscopic visualization of biocytin or neurobiotin-labeled neurons
Authors: Sun XJ, Tolbert LP, Hildebr and JG, Meinertzhagen IA.
Journal: J Histochem Cytochem (1998): 263
A radioiodinated biocytin derivative for in-vivo applications
Authors: Nakamoto Y, Sakahara H, Saga T, Yao Z, Zhang M, Sato N, Zhao S, Arano Y, Ono M, Saji H, Konishi J.
Journal: Nucl Med Commun (1998): 963
Fluorophore-linked assays for high-performance liquid chromatography postcolumn reaction detection of biotin and biocytin
Authors: Hentz NG, Bachas LG.
Journal: Methods Enzymol (1997): 275