Biotin-16-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 136632-31-0*

Biotin-16-dUTP

1 mM in TE Buffer (pH 7.5); CAS 136632-31-0

The biotin-modified deoxyuridine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3'-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-16-dUTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '16' is the number of carbon atoms in the backbone of the linker between dUTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dUTP into DNA. Biotin-16-dUTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells and tissues.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17017	25 nmoles	Price

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

Authors:

Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.

Journal:

Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

Authors:

Cox WG, Singer VL.

Journal:

Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking

Authors:

Wooddell CI, Burgess RR.

Journal:

Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP

Authors:

Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.

Journal:

Biotechniques (2000): 518

Physical properties

Molecular weight	947.78
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	136632-31-0

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 28, 2025