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Biotin Azide

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight326.42
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Alternative formats
Biotin cadaverine
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OverviewpdfSDSpdfProtocol


Molecular weight
326.42
Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins. Biotin azide can be readily conjugated to many biomolecules through the well-known click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM306.354 µL1.532 mL3.064 mL15.318 mL30.635 mL
5 mM61.271 µL306.354 µL612.708 µL3.064 mL6.127 mL
10 mM30.635 µL153.177 µL306.354 µL1.532 mL3.064 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Citations


View all 3 citations: Citation Explorer
A low-cost, high-performance system for fluorescence lateral flow assays
Authors: Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F
Journal: Biosensors (2013): 360--373
Implementation of P22 viral capsids as nanoplatforms
Authors: Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor
Journal: Biomacromolecules (2010): 2804--2809
Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields
Authors: Morotomi-Yano, Keiko and Yano, Ken-ichi
Journal: FEBS Open Bio

References


View all 100 references: Citation Explorer
Selective Removal of Unhydrolyzed Monolinked Peptides from Enriched Crosslinked Peptides To Improve the Coverage of Protein Complex Analysis.
Authors: An, Yuxin and Zhao, Qun and Gao, Hang and Zhao, Lili and Li, Xiao and Zhang, Xiaodan and Liang, Zhen and Zhang, Lihua and Zhang, Yukui
Journal: Analytical chemistry (2022): 3904-3913
Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy.
Authors: Tsuji, Takuma and Fujimoto, Toyoshi
Journal: STAR protocols (2021): 100990
Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
Maleimide-Based Chemical Proteomics for Quantitative Analysis of Cysteine Reactivity.
Authors: McConnell, Evan W and Smythers, Amanda L and Hicks, Leslie M
Journal: Journal of the American Society for Mass Spectrometry (2020)
Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.
Authors: Flaxman, Hope A and Miyamoto, David K and Woo, Christina M
Journal: Current protocols in chemical biology (2019): e75
Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP).
Authors: Dadfar, Seyed Mohammad Mahdi and Sekula-Neuner, Sylwia and Trouillet, Vanessa and Liu, Hui-Yu and Kumar, Ravi and Powell, Annie K and Hirtz, Michael
Journal: Beilstein journal of nanotechnology (2019): 2505-2515
Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.
Authors: Sobocińska, Justyna and Roszczenko-Jasińska, Paula and Zaręba-Kozioł, Monika and Hromada-Judycka, Aneta and Matveichuk, Orest V and Traczyk, Gabriela and Łukasiuk, Katarzyna and Kwiatkowska, Katarzyna
Journal: Molecular & cellular proteomics : MCP (2018): 233-254
Single-Protein-Specific Redox Targeting in Live Mammalian Cells and C. elegans.
Authors: Van Hall-Beauvais, Alexandra and Zhao, Yi and Urul, Daniel A and Long, Marcus J C and Aye, Yimon
Journal: Current protocols in chemical biology (2018): e43
Revealing nascent proteomics in signaling pathways and cell differentiation.
Authors: Forester, Craig M and Zhao, Qian and Phillips, Nancy J and Urisman, Anatoly and Chalkley, Robert J and Oses-Prieto, Juan A and Zhang, Li and Ruggero, Davide and Burlingame, Alma L
Journal: Proceedings of the National Academy of Sciences of the United States of America (2018): 2353-2358
A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein-Protein Interactions.
Authors: Joiner, Cassandra M and Breen, Meghan E and Clayton, James and Mapp, Anna K
Journal: Chembiochem : a European journal of chemical biology (2017): 181-184