Biotin Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	326.42
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

326.42

Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins. Biotin azide can be readily conjugated to many biomolecules through the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	306.354 µL	1.532 mL	3.064 mL	15.318 mL	30.635 mL
5 mM	61.271 µL	306.354 µL	612.708 µL	3.064 mL	6.127 mL
10 mM	30.635 µL	153.177 µL	306.354 µL	1.532 mL	3.064 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for Biotin Azide.

Citations

View all 3 citations: Citation Explorer

A low-cost, high-performance system for fluorescence lateral flow assays
Authors: Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F
Journal: Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms
Authors: Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor
Journal: Biomacromolecules (2010): 2804--2809

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields
Authors: Morotomi-Yano, Keiko and Yano, Ken-ichi
Journal: FEBS Open Bio

References

View all 100 references: Citation Explorer

Selective Removal of Unhydrolyzed Monolinked Peptides from Enriched Crosslinked Peptides To Improve the Coverage of Protein Complex Analysis.
Authors: An, Yuxin and Zhao, Qun and Gao, Hang and Zhao, Lili and Li, Xiao and Zhang, Xiaodan and Liang, Zhen and Zhang, Lihua and Zhang, Yukui
Journal: Analytical chemistry (2022): 3904-3913

Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy.
Authors: Tsuji, Takuma and Fujimoto, Toyoshi
Journal: STAR protocols (2021): 100990

Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213

Maleimide-Based Chemical Proteomics for Quantitative Analysis of Cysteine Reactivity.
Authors: McConnell, Evan W and Smythers, Amanda L and Hicks, Leslie M
Journal: Journal of the American Society for Mass Spectrometry (2020)

Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.
Authors: Flaxman, Hope A and Miyamoto, David K and Woo, Christina M
Journal: Current protocols in chemical biology (2019): e75

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP).
Authors: Dadfar, Seyed Mohammad Mahdi and Sekula-Neuner, Sylwia and Trouillet, Vanessa and Liu, Hui-Yu and Kumar, Ravi and Powell, Annie K and Hirtz, Michael
Journal: Beilstein journal of nanotechnology (2019): 2505-2515

Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.
Authors: Sobocińska, Justyna and Roszczenko-Jasińska, Paula and Zaręba-Kozioł, Monika and Hromada-Judycka, Aneta and Matveichuk, Orest V and Traczyk, Gabriela and Łukasiuk, Katarzyna and Kwiatkowska, Katarzyna
Journal: Molecular & cellular proteomics : MCP (2018): 233-254

Single-Protein-Specific Redox Targeting in Live Mammalian Cells and C. elegans.
Authors: Van Hall-Beauvais, Alexandra and Zhao, Yi and Urul, Daniel A and Long, Marcus J C and Aye, Yimon
Journal: Current protocols in chemical biology (2018): e43

Revealing nascent proteomics in signaling pathways and cell differentiation.
Authors: Forester, Craig M and Zhao, Qian and Phillips, Nancy J and Urisman, Anatoly and Chalkley, Robert J and Oses-Prieto, Juan A and Zhang, Li and Ruggero, Davide and Burlingame, Alma L
Journal: Proceedings of the National Academy of Sciences of the United States of America (2018): 2353-2358

A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein-Protein Interactions.
Authors: Joiner, Cassandra M and Breen, Meghan E and Clayton, James and Mapp, Anna K
Journal: Chembiochem : a European journal of chemical biology (2017): 181-184