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AF532 PEG4 biotin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1172.48 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.24 |
| Correction Factor (280 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 81000 |
| Excitation (nm) | 534 |
| Emission (nm) | 553 |
| Quantum yield | 0.611 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Biotin and Streptavidin
Molecular weight 1172.48 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 534 | Emission (nm) 553 | Quantum yield 0.611 |
AF532 PEG4 biotin conjugate contains Alexa Fluor 532 fluorophore that is well excited by the common 532 nm laser. It demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. AF532 PEG4 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. AF532 PEG4 biotin conjugate is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of AF532 PEG4 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 85.289 µL | 426.447 µL | 852.893 µL | 4.264 mL | 8.529 mL |
| 5 mM | 17.058 µL | 85.289 µL | 170.579 µL | 852.893 µL | 1.706 mL |
| 10 mM | 8.529 µL | 42.645 µL | 85.289 µL | 426.447 µL | 852.893 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.24 |
| Correction Factor (280 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 81000 |
| Excitation (nm) | 534 |
| Emission (nm) | 553 |
| Quantum yield | 0.611 |
Images
Figure 1. Chemical structure for AF532 PEG4 biotin conjugate.
References
View all 47 references: Citation Explorer
An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates.
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots.
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Integration of Enzymatic Labeling with Single-Molecule Detection for Sensitive Quantification of Diverse DNA Damages.
Authors: Zhang, Yan and Hua, Ruo-Nan and Zhang, Chun-Yang
Journal: Analytical chemistry (2020): 4700-4706
Authors: Zhang, Yan and Hua, Ruo-Nan and Zhang, Chun-Yang
Journal: Analytical chemistry (2020): 4700-4706
A Vector-Based Method to Analyze the Topography of Glial Networks.
Authors: Eitelmann, Sara and Hirtz, Jan J and Stephan, Jonathan
Journal: International journal of molecular sciences (2019)
Authors: Eitelmann, Sara and Hirtz, Jan J and Stephan, Jonathan
Journal: International journal of molecular sciences (2019)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Enhanced broadband fluorescence detection of nucleic acids using multipolar gap-plasmons on biomimetic Au metasurfaces.
Authors: Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck
Journal: Nanoscale (2019): 13750-13757
Authors: Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck
Journal: Nanoscale (2019): 13750-13757
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74