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AF532 PEG4 biotin conjugate

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Physical properties
Molecular weight1172.48
SolventWater
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)534
Emission (nm)553
Quantum yield0.611
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
1172.48
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.09
Extinction coefficient (cm -1 M -1)
81000
Excitation (nm)
534
Emission (nm)
553
Quantum yield
0.611
AF532 PEG4 biotin conjugate contains Alexa Fluor 532 fluorophore that is well excited by the common 532 nm laser. It demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. AF532 PEG4 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. AF532 PEG4 biotin conjugate is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of AF532 PEG4 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM85.289 µL426.447 µL852.893 µL4.264 mL8.529 mL
5 mM17.058 µL85.289 µL170.579 µL852.893 µL1.706 mL
10 mM8.529 µL42.645 µL85.289 µL426.447 µL852.893 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)534
Emission (nm)553
Quantum yield0.611

Images


References


View all 47 references: Citation Explorer
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates.
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots.
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Integration of Enzymatic Labeling with Single-Molecule Detection for Sensitive Quantification of Diverse DNA Damages.
Authors: Zhang, Yan and Hua, Ruo-Nan and Zhang, Chun-Yang
Journal: Analytical chemistry (2020): 4700-4706
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
A Vector-Based Method to Analyze the Topography of Glial Networks.
Authors: Eitelmann, Sara and Hirtz, Jan J and Stephan, Jonathan
Journal: International journal of molecular sciences (2019)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Enhanced broadband fluorescence detection of nucleic acids using multipolar gap-plasmons on biomimetic Au metasurfaces.
Authors: Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck
Journal: Nanoscale (2019): 13750-13757