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Acridinium Biotin Conjugate

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Physical properties
Molecular weight928.03
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Acridinium biotin is a conjugate of acridinium ester and biotin. It is a chemiluminescent label that can be used to detect antibodies or antigens in a wide range of immunoassays. For example, it may be used to label antibodies or antigens that can be detected by streptavidin-coated magnetic beads in a chemiluminescent immunoassay system. Since biotin is a small molecule that can bind to streptavidin with high affinity and specificity, the acridinium biotin reagent can be used in a variety of immunological applications in combination of numerous commercially available streptavidin conjugates.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM107.755 µL538.776 µL1.078 mL5.388 mL10.776 mL
5 mM21.551 µL107.755 µL215.51 µL1.078 mL2.155 mL
10 mM10.776 µL53.878 µL107.755 µL538.776 µL1.078 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy5 biotin conjugate65167025000010.271, 0.420.020.03
Cy3 biotin conjugate55556915000010.1510.070.073
Cy7 biotin conjugate7567792500000.30.050.036



View all 13 references: Citation Explorer
Magnetic Nanoparticle-Based Automatic Chemiluminescent Enzyme Immunoassay for Golgi Protein 73 and the Clinical Assessment.
Authors: Wei, Xuefei and Xia, Yanyan and Shen, Mengjiao and Yang, Yue and Jin, Jing and Xu, Hongpan and Li, Zhiyang
Journal: Journal of nanoscience and nanotechnology (2019): 1971-1977
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
Authors: Reily, Colin and Rizk, Dana V and Julian, Bruce A and Novak, Jan
Journal: BioTechniques (2018): 71-77
A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
Authors: Yin, Dongguang and Cui, Daxiang and Gao, Feng and He, Rong and He, Youfeng and Liu, Yibing and Shen, Decun and Wu, Minghong
Journal: Journal of immunoassay & immunochemistry (2008): 257-65
Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.
Authors: Adamczyk, Maciej and Mattingly, Phillip G and Moore, Jeffrey A and Pan, You
Journal: Organic letters (2003): 3779-82
Single-nucleotide polymorphism analysis by hybridization protection assay on solid support.
Authors: Goto, Susumu and Takahashi, Aki and Kamisango, Keiichi and Matsubara, Koichi
Journal: Analytical biochemistry (2002): 25-32
Region-selective labeling of antibodies as determined by electrospray ionization-mass spectrometry (ESI-MS).
Authors: Adamczyk, M and Gebler, J and Shreder, K and Wu, J
Journal: Bioconjugate chemistry (2000): 557-63
Surface plasmon resonance (SPR) as a tool for antibody conjugate analysis.
Authors: Adamczyk, M and Mattingly, P G and Shreder, K and Yu, Z
Journal: Bioconjugate chemistry (1999): 1032-7
Resin-supported labeling reagents.
Authors: Adamczyk, M and Fishpaugh, J R and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1999): 217-20
Estradiol-mimetic probes. Preparation of 17 alpha-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates.
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4
Synthesis of 6 beta-aminoestradiol and its biotin, acridinium, and fluorescein conjugates.
Authors: Adamczyk, M and Mattingly, P G and Reddy, R E
Journal: Steroids (1998): 130-4