Acridinium Biotin Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 928.03 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
See also: Biotin and Streptavidin
Molecular weight 928.03 |
Acridinium biotin is a conjugate of acridinium ester and biotin. It is a chemiluminescent label that can be used to detect antibodies or antigens in a wide range of immunoassays. For example, it may be used to label antibodies or antigens that can be detected by streptavidin-coated magnetic beads in a chemiluminescent immunoassay system. Since biotin is a small molecule that can bind to streptavidin with high affinity and specificity, the acridinium biotin reagent can be used in a variety of immunological applications in combination of numerous commercially available streptavidin conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 107.755 µL | 538.776 µL | 1.078 mL | 5.388 mL | 10.776 mL |
| 5 mM | 21.551 µL | 107.755 µL | 215.51 µL | 1.078 mL | 2.155 mL |
| 10 mM | 10.776 µL | 53.878 µL | 107.755 µL | 538.776 µL | 1.078 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy5 biotin conjugate | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| Cy3 biotin conjugate | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy7 biotin conjugate | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
Images
Figure 1. Acridinium biotin is a conjugate of acridinium ester and biotin. It is a chemiluminescent label that can be used to detect antibodies or antigens in a wide range of immunoassays.
References
View all 13 references: Citation Explorer
Magnetic Nanoparticle-Based Automatic Chemiluminescent Enzyme Immunoassay for Golgi Protein 73 and the Clinical Assessment.
Authors: Wei, Xuefei and Xia, Yanyan and Shen, Mengjiao and Yang, Yue and Jin, Jing and Xu, Hongpan and Li, Zhiyang
Journal: Journal of nanoscience and nanotechnology (2019): 1971-1977
Authors: Wei, Xuefei and Xia, Yanyan and Shen, Mengjiao and Yang, Yue and Jin, Jing and Xu, Hongpan and Li, Zhiyang
Journal: Journal of nanoscience and nanotechnology (2019): 1971-1977
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
Authors: Reily, Colin and Rizk, Dana V and Julian, Bruce A and Novak, Jan
Journal: BioTechniques (2018): 71-77
Authors: Reily, Colin and Rizk, Dana V and Julian, Bruce A and Novak, Jan
Journal: BioTechniques (2018): 71-77
A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
Authors: Yin, Dongguang and Cui, Daxiang and Gao, Feng and He, Rong and He, Youfeng and Liu, Yibing and Shen, Decun and Wu, Minghong
Journal: Journal of immunoassay & immunochemistry (2008): 257-65
Authors: Yin, Dongguang and Cui, Daxiang and Gao, Feng and He, Rong and He, Youfeng and Liu, Yibing and Shen, Decun and Wu, Minghong
Journal: Journal of immunoassay & immunochemistry (2008): 257-65
Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.
Authors: Adamczyk, Maciej and Mattingly, Phillip G and Moore, Jeffrey A and Pan, You
Journal: Organic letters (2003): 3779-82
Authors: Adamczyk, Maciej and Mattingly, Phillip G and Moore, Jeffrey A and Pan, You
Journal: Organic letters (2003): 3779-82
Single-nucleotide polymorphism analysis by hybridization protection assay on solid support.
Authors: Goto, Susumu and Takahashi, Aki and Kamisango, Keiichi and Matsubara, Koichi
Journal: Analytical biochemistry (2002): 25-32
Authors: Goto, Susumu and Takahashi, Aki and Kamisango, Keiichi and Matsubara, Koichi
Journal: Analytical biochemistry (2002): 25-32
Region-selective labeling of antibodies as determined by electrospray ionization-mass spectrometry (ESI-MS).
Authors: Adamczyk, M and Gebler, J and Shreder, K and Wu, J
Journal: Bioconjugate chemistry (2000): 557-63
Authors: Adamczyk, M and Gebler, J and Shreder, K and Wu, J
Journal: Bioconjugate chemistry (2000): 557-63
Surface plasmon resonance (SPR) as a tool for antibody conjugate analysis.
Authors: Adamczyk, M and Mattingly, P G and Shreder, K and Yu, Z
Journal: Bioconjugate chemistry (1999): 1032-7
Authors: Adamczyk, M and Mattingly, P G and Shreder, K and Yu, Z
Journal: Bioconjugate chemistry (1999): 1032-7
Resin-supported labeling reagents.
Authors: Adamczyk, M and Fishpaugh, J R and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1999): 217-20
Authors: Adamczyk, M and Fishpaugh, J R and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1999): 217-20
Estradiol-mimetic probes. Preparation of 17 alpha-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates.
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4
Synthesis of 6 beta-aminoestradiol and its biotin, acridinium, and fluorescein conjugates.
Authors: Adamczyk, M and Mattingly, P G and Reddy, R E
Journal: Steroids (1998): 130-4
Authors: Adamczyk, M and Mattingly, P G and Reddy, R E
Journal: Steroids (1998): 130-4