Acridinium Biotin Conjugate

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	928.03
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.755 µL	538.776 µL	1.078 mL	5.388 mL	10.776 mL
5 mM	21.551 µL	107.755 µL	215.51 µL	1.078 mL	2.155 mL
10 mM	10.776 µL	53.878 µL	107.755 µL	538.776 µL	1.078 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Acridinium biotin is a conjugate of acridinium ester and biotin. It is a chemiluminescent label that can be used to detect antibodies or antigens in a wide range of immunoassays.

References

View all 13 references: Citation Explorer

Magnetic Nanoparticle-Based Automatic Chemiluminescent Enzyme Immunoassay for Golgi Protein 73 and the Clinical Assessment.
Authors: Wei, Xuefei and Xia, Yanyan and Shen, Mengjiao and Yang, Yue and Jin, Jing and Xu, Hongpan and Li, Zhiyang
Journal: Journal of nanoscience and nanotechnology (2019): 1971-1977

Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
Authors: Reily, Colin and Rizk, Dana V and Julian, Bruce A and Novak, Jan
Journal: BioTechniques (2018): 71-77

A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
Authors: Yin, Dongguang and Cui, Daxiang and Gao, Feng and He, Rong and He, Youfeng and Liu, Yibing and Shen, Decun and Wu, Minghong
Journal: Journal of immunoassay & immunochemistry (2008): 257-65

Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin.
Authors: Adamczyk, Maciej and Mattingly, Phillip G and Moore, Jeffrey A and Pan, You
Journal: Organic letters (2003): 3779-82

Single-nucleotide polymorphism analysis by hybridization protection assay on solid support.
Authors: Goto, Susumu and Takahashi, Aki and Kamisango, Keiichi and Matsubara, Koichi
Journal: Analytical biochemistry (2002): 25-32

Region-selective labeling of antibodies as determined by electrospray ionization-mass spectrometry (ESI-MS).
Authors: Adamczyk, M and Gebler, J and Shreder, K and Wu, J
Journal: Bioconjugate chemistry (2000): 557-63

Surface plasmon resonance (SPR) as a tool for antibody conjugate analysis.
Authors: Adamczyk, M and Mattingly, P G and Shreder, K and Yu, Z
Journal: Bioconjugate chemistry (1999): 1032-7

Resin-supported labeling reagents.
Authors: Adamczyk, M and Fishpaugh, J R and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1999): 217-20

Estradiol-mimetic probes. Preparation of 17 alpha-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates.
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4

Synthesis of 6 beta-aminoestradiol and its biotin, acridinium, and fluorescein conjugates.
Authors: Adamczyk, M and Mattingly, P G and Reddy, R E
Journal: Steroids (1998): 130-4