Biotin-dT Phosphoramidite
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1285.55 |
Solvent | MeCN |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Biotin and Streptavidin
CAS 198080-40-9 | Molecular weight 1285.55 |
Biotin-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the biotin tag to a desired location of an oligo sequence. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin-dT phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer FAM-dT phosphoramidite, HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of Biotin-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 77.788 µL | 388.939 µL | 777.877 µL | 3.889 mL | 7.779 mL |
5 mM | 15.558 µL | 77.788 µL | 155.575 µL | 777.877 µL | 1.556 mL |
10 mM | 7.779 µL | 38.894 µL | 77.788 µL | 388.939 µL | 777.877 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
HEX-dT Phosphoramidite | 533 | 559 | 73000 | 0.15 |
Fluorescein-dT Phosphoramidite | 498 | 517 | 800001 | 0.35 |
References
View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6