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Biotin-dT Phosphoramidite

Chemical structure for Biotin-dT Phosphoramidite.
Chemical structure for Biotin-dT Phosphoramidite.
Ordering information
Price ()
Catalog Number6220
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1285.55
SolventMeCN
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Biotin Phosphoramidite
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OverviewpdfSDSpdfProtocol


CAS
198080-40-9
Molecular weight
1285.55
Biotin-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the biotin tag to a desired location of an oligo sequence. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin-dT phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer FAM-dT phosphoramidite, HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.

Calculators


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Biotin-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.788 µL388.939 µL777.877 µL3.889 mL7.779 mL
5 mM15.558 µL77.788 µL155.575 µL777.877 µL1.556 mL
10 mM7.779 µL38.894 µL77.788 µL388.939 µL777.877 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6