Biotin-dT Phosphoramidite
| Overview |  SDSProtocol |
See also: Bioconjugation, Biotin and Streptavidin
CAS 198080-40-9 | Molecular weight 1285.55 |
Biotin-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the biotin tag to a desired location of an oligo sequence. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin-dT phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer FAM-dT phosphoramidite, HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of Biotin-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 77.788 µL | 388.939 µL | 777.877 µL | 3.889 mL | 7.779 mL |
| 5 mM | 15.558 µL | 77.788 µL | 155.575 µL | 777.877 µL | 1.556 mL |
| 10 mM | 7.779 µL | 38.894 µL | 77.788 µL | 388.939 µL | 777.877 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for Biotin-dT Phosphoramidite.
References
View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6