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Biotin NTA
Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol. NTA Biotin is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The NTA-polyHis-complex can be detected using standard enzyme-linked streptavidin methods, such as a streptavidin-horseradish peroxidase conjugate. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe. In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins.
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 3008 | 5 mg | Price |
Physical properties
| Molecular weight | 488.56 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| CAS | 176046-06-3 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 22, 2025