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Biotin NTA

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Physical properties
Molecular weight488.56
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


CAS
176046-06-3
Molecular weight
488.56
Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol. NTA Biotin is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The NTA-polyHis-complex can be detected using standard enzyme-linked streptavidin methods, such as a streptavidin-horseradish peroxidase conjugate. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe. In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin NTA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM204.683 µL1.023 mL2.047 mL10.234 mL20.468 mL
5 mM40.937 µL204.683 µL409.366 µL2.047 mL4.094 mL
10 mM20.468 µL102.342 µL204.683 µL1.023 mL2.047 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 2 references: Citation Explorer
Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer.
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449
Magnetically enhanced adeno-associated viral vector delivery for human neural stem cell infection.
Authors: Kim, Eunmi and Oh, Ji-Seon and Ahn, Ik-Sung and Park, Kook In and Jang, Jae-Hyung
Journal: Biomaterials (2011): 8654-62