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Biotin NTA

Chemical structure for Biotin NTA .
Chemical structure for Biotin NTA .
Ordering information
Price ()
Catalog Number3008
Unit Size
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Physical properties
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


CAS
176046-06-3
Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol. NTA Biotin is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The NTA-polyHis-complex can be detected using standard enzyme-linked streptavidin methods, such as a streptavidin-horseradish peroxidase conjugate. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe. In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins.

References


View all 2 references: Citation Explorer
Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer.
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449
Magnetically enhanced adeno-associated viral vector delivery for human neural stem cell infection.
Authors: Kim, Eunmi and Oh, Ji-Seon and Ahn, Ik-Sung and Park, Kook In and Jang, Jae-Hyung
Journal: Biomaterials (2011): 8654-62