Biotin NTA
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 488.56 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
See also: Biotin and Streptavidin
CAS 176046-06-3 | Molecular weight 488.56 |
Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol. NTA Biotin is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The NTA-polyHis-complex can be detected using standard enzyme-linked streptavidin methods, such as a streptavidin-horseradish peroxidase conjugate. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe. In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin NTA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 204.683 µL | 1.023 mL | 2.047 mL | 10.234 mL | 20.468 mL |
5 mM | 40.937 µL | 204.683 µL | 409.366 µL | 2.047 mL | 4.094 mL |
10 mM | 20.468 µL | 102.342 µL | 204.683 µL | 1.023 mL | 2.047 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 2 references: Citation Explorer
Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer.
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449
Authors: Gunnarsson, Anders and Simonsson Nyström, Lisa and Burazerovic, Sabina and Gunnarsson, Jenny and Snijder, Arjan and Geschwindner, Stefan and Höök, Fredrik
Journal: ChemistryOpen (2016): 445-449
Magnetically enhanced adeno-associated viral vector delivery for human neural stem cell infection.
Authors: Kim, Eunmi and Oh, Ji-Seon and Ahn, Ik-Sung and Park, Kook In and Jang, Jae-Hyung
Journal: Biomaterials (2011): 8654-62
Authors: Kim, Eunmi and Oh, Ji-Seon and Ahn, Ik-Sung and Park, Kook In and Jang, Jae-Hyung
Journal: Biomaterials (2011): 8654-62