Biotin PEG3 amine

Biotin PEG3 amine is a carbonyl-reactive biotin derivative that contains a long arm to increase its avidin-binding affinity. This PEG biotin reacts specifically and efficiently with carboxy (e.g., a side chain of Glu or Asp residues or carboxy-coated surfaces) to form covalent bonds. The PEG spacer arm gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. It is an excelllent building block that can be used to label a variety of biological molecules and samples.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3024	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin PEG3 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	178.371 µL	891.854 µL	1.784 mL	8.919 mL	17.837 mL
5 mM	35.674 µL	178.371 µL	356.742 µL	1.784 mL	3.567 mL
10 mM	17.837 µL	89.185 µL	178.371 µL	891.854 µL	1.784 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	560.63 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

A low-cost, high-performance system for fluorescence lateral flow assays

Authors:

Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F

Journal:

Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms

Authors:

Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor

Journal:

Biomacromolecules (2010): 2804--2809

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields

Authors:

Morotomi-Yano, Keiko and Yano, Ken-ichi

Journal:

FEBS Open Bio

References

View all 78 references: Citation Explorer

Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))

Authors:

Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.

Journal:

Eur J Nucl Med Mol Imaging. (2006)

A biotin-protein bond with stability in plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2005): 98

Instability of the biotin-protein bond in human plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2004): 156

Design, synthesis, and evaluation of 5'-S-aminoethyl-N(6)- azidobenzyl-5'-thioadenosine biotin conjugate: a bifunctional photoaffinity probe for the es nucleoside transporter

Authors:

Addo JK, Buolamwini JK.

Journal:

Bioconjug Chem (2004): 536

Biotin is endogenously expressed in select regions of the rat central nervous system

Authors:

McKay BE, Molineux ML, Turner RW.

Journal:

J Comp Neurol (2004): 86

Physical properties

Molecular weight	560.63
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200
CAS	194920-43-9

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Page updated on October 13, 2025