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Biotin Phosphoramidite

Biotin phosphoramidite contains a DMT group to allow quantification of coupling. It is soluble in acetonitrile, providing a valuable tool to add the biotin tag to a 3’ or 5’ location of an oligo sequence. Biotin phosphoramidite contains a 15-atom spacer arm minimizing the potential hindrance of biotin binding.
Biotin phosphoramidite contains a DMT group to allow quantification of coupling. It is soluble in acetonitrile, providing a valuable tool to add the biotin tag to a 3’ or 5’ location of an oligo sequence. Biotin phosphoramidite contains a 15-atom spacer arm minimizing the potential hindrance of biotin binding.
Ordering information
Price ()
Catalog Number6222
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight876.11
SolventMeCN
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


CAS
147190-34-9
Molecular weight
876.11
Biotin phosphoramidite contains a DMT group to allow quantification of coupling. It is soluble in acetonitrile, providing a valuable tool to add the biotin tag to a 3’ or 5’ location of an oligo sequence. Biotin phosphoramidite contains a 15-atom spacer arm minimizing the potential hindrance of biotin binding. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer biotin-dT phosphoramidite and biotin-dT CPG.

Calculators


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Biotin Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM114.141 µL570.705 µL1.141 mL5.707 mL11.414 mL
5 mM22.828 µL114.141 µL228.282 µL1.141 mL2.283 mL
10 mM11.414 µL57.07 µL114.141 µL570.705 µL1.141 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6