Biotin Phosphoramidite
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 876.11 |
Solvent | MeCN |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
See also: Biotin and Streptavidin
CAS 147190-34-9 | Molecular weight 876.11 |
Biotin phosphoramidite contains a DMT group to allow quantification of coupling. It is soluble in acetonitrile, providing a valuable tool to add the biotin tag to a 3’ or 5’ location of an oligo sequence. Biotin phosphoramidite contains a 15-atom spacer arm minimizing the potential hindrance of biotin binding. It is complimentary to the commonly used biotin phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. The DMT group on biotin phosphoramidite can be used in RP cartridge and HPLC purification techniques. AAT Bioquest also offer biotin-dT phosphoramidite and biotin-dT CPG.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of Biotin Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 114.141 µL | 570.705 µL | 1.141 mL | 5.707 mL | 11.414 mL |
5 mM | 22.828 µL | 114.141 µL | 228.282 µL | 1.141 mL | 2.283 mL |
10 mM | 11.414 µL | 57.07 µL | 114.141 µL | 570.705 µL | 1.141 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Biotin phosphoramidite contains a DMT group to allow quantification of coupling. It is soluble in acetonitrile, providing a valuable tool to add the biotin tag to a 3’ or 5’ location of an oligo sequence. Biotin phosphoramidite contains a 15-atom spacer arm minimizing the potential hindrance of biotin binding.
References
View all 1 references: Citation Explorer
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6
Authors: Olejnik, J and Krzymańska-Olejnik, E and Rothschild, K J
Journal: Nucleic acids research (1996): 361-6