Biotin, succinimidyl ester *CAS 35013-72-0*

Biotin, succinimidyl ester

CAS 35013-72-0

Biotin succinimidyl ester is the most popular amine-reactive biotin derivative for modifying proteins and other biological molecules. This primary amine coupling reagent is successfully used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3002	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin, succinimidyl ester *CAS 35013-72-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	292.929 µL	1.465 mL	2.929 mL	14.646 mL	29.293 mL
5 mM	58.586 µL	292.929 µL	585.857 µL	2.929 mL	5.859 mL
10 mM	29.293 µL	146.464 µL	292.929 µL	1.465 mL	2.929 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	341.38 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

A low-cost, high-performance system for fluorescence lateral flow assays

Authors:

Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F

Journal:

Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms

Authors:

Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor

Journal:

Biomacromolecules (2010): 2804--2809

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields

Authors:

Morotomi-Yano, Keiko and Yano, Ken-ichi

Journal:

FEBS Open Bio

References

View all 78 references: Citation Explorer

Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))

Authors:

Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.

Journal:

Eur J Nucl Med Mol Imaging. (2006)

A biotin-protein bond with stability in plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2005): 98

Instability of the biotin-protein bond in human plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2004): 156

Design, synthesis, and evaluation of 5'-S-aminoethyl-N(6)- azidobenzyl-5'-thioadenosine biotin conjugate: a bifunctional photoaffinity probe for the es nucleoside transporter

Authors:

Addo JK, Buolamwini JK.

Journal:

Bioconjug Chem (2004): 536

Biotin is endogenously expressed in select regions of the rat central nervous system

Authors:

McKay BE, Molineux ML, Turner RW.

Journal:

J Comp Neurol (2004): 86

Physical properties

Molecular weight	341.38
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200
CAS	35013-72-0

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