Name: Biotin-xtraFluor™ Violet 420 Conjugate
Brand: AAT Bioquest
SKU: 70804
Price: 199 USD
Availability: InStock

Biotin-xtraFluor™ Violet 420 Conjugate

Biotin XtraFluor™ Violet 420 Conjugate is an ultra-bright violet-excitable fluorescent biotin derivative optimized for 405 nm laser excitation, providing superior brightness for streptavidin-based detection in multicolor flow cytometry applications.

Spectral profile: Ex/Em ≈ 405/420 nm; optimized for the 405 nm violet laser
Superior Brightness: Brighter than Pacific Blue (ThermoFisher) and BD Horizon™ V450 (BD Biosciences)
Multiplexing: Minimal spillover into Pacific Orange™ and Brilliant Violet 510™ channels
Biotin functionality: Fluorescent biotin derivative for streptavidin/avidin-based detection workflows

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
70804	100 ug	Price

Overview

Biotin-xtraFluor™ Violet 420 Conjugate might be the brightest biotin dye conjugate commercially available. It can be conveniently used for detecting avidin-tagged biomolecules. XV420 is an extremely bright violet laser-excitable fluorophore. With a maximum excitation well matched the 405 nm violet laser and an emission peak at ~420 nm, XV420 can be conveniently used to replace BD Horizon Brilliant™ Violet 421 (BV421). It can be used on flow cytometers equipped with a violet laser and appropriate filters for large multicolor flow cytometry panels. It is a brighter alternative to Pacific Blue (ThermoFisher) and BD Horizon™ V450 (BD Biosciences). XV420 enables improved resolution for dim population identification. It is particularly useful in multicolor applications where it can be used to better resolve dim populations. XV420 dye offers new options for multicolor panel design through the use of a violet laser. Additionally, the dye’s spillover properties, stability under light and in standard buffers, and compatibility with blood collection tubes offer ease-of-use for a range of applications.

References

View all 49 references: Citation Explorer

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.

Authors:

Sahi, Maryam and Andersson, Sarah and Mattson, Cecilia and Dale, Matilda and Kagiolglou, Sofia and Hofström, Camilla and Persson, Helena and Klingström, Jonas and Chiodi, Francesca and Fredolini, Claudia

Journal:

Journal of visualized experiments : JoVE (2024)

Effects of solid retention time and exposure mode to electric current on Remazol Brilliant Violet removal in an electro-membrane bioreactor.

Authors:

Belli, Tiago José and Bassin, João Paulo and de Sousa Vidal, Carlos Magno and Hassemer, Maria Eliza Nagel and Rodrigues, Caroline and Lapolli, Flávio Rubens

Journal:

Environmental science and pollution research international (2023): 58412-58427

Sonophotocatalytic Degradation of Fast Yellow AB and Remazol Brilliant Violet-5R by Using Ag-Impregnated ZnO as a Photocatalyst.

Authors:

Aziz, Nosheen and Rasool, Shagufta and Ullah, Toheed and Khitab, Fatima and Halim, Sobia Ahsan and Bawazeer, Sami and Khan, Ajmal and Al-Harrasi, Ahmed

Journal:

ACS omega (2023): 18509-18515

Performance of treatment schemes comprising chromium-hydrogen peroxide-based advanced oxidation process for textile wastewater.

Authors:

Mondal, Prasenjit and Mukherji, Suparna and Garg, Anurag

Journal:

Environmental science and pollution research international (2022): 88089-88100

Comparative study and dyeing performance of as-synthesized azo heterocyclic monomeric, polymeric, and commercial disperse dyes.

Authors:

Abdullahi, Shehu Sa'ad and Musa, Haruna and Habibu, Shehu and Birniwa, Abdullahi Haruna and Mohammad, Rania Edrees Adam

Journal:

Turkish journal of chemistry (2022): 1841-1852

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (280 nm)	0.067
Extinction coefficient (cm ^-1 M ^-1)	3,000,000
Excitation (nm)	407
Emission (nm)	424

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on April 6, 2026