BiotinTEG Phosphoramidite is used for site-specific incorporation of biotin into oligonucleotides during solid-phase synthesis. This reagent consists of a biotin moiety linked to a phosphoramidite functional group via a triethylene glycol (TEG) spacer. The TEG linker enhances solubility and conformational flexibility while minimizing steric hindrance, allowing for more efficient coupling and improved accessibility of the biotin group. This phosphoramidite is compatible with standard DNA and RNA synthesis chemistries and instrumentation, enabling reproducible 5′-end or internal biotin labeling under conventional coupling and deprotection conditions.

Biotin TEG Phosphoramidite is widely used for the synthesis of biotinylated oligonucleotides applied in affinity-based and analytical assays such as ELISA, surface plasmon resonance (SPR), quantitative PCR (qPCR), microarray hybridization, and bead-based capture systems. The hydrophilic, extended TEG spacer enhances the functional performance of biotin–streptavidin and related interactions by reducing steric constraints and ensuring optimal biotin presentation in downstream hybridization, immobilization, and biosensing applications.