Bis-Arg-rhodamine 110
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1098.81 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Proteases
Molecular weight 1098.81 | Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 500 | Emission (nm) 522 |
This Bis Arg-rhodamine 110 is a fluorogenic aminopeptidase and endopeptidase substrate used for the detection of enzymatic activity of human alpha-thrombin. The non-fluorescent substrate generates a bright green fluorescent rhodamine product, and can be easily detected with the FITC filter set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Bis-Arg-rhodamine 110 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 91.008 µL | 455.038 µL | 910.075 µL | 4.55 mL | 9.101 mL |
5 mM | 18.202 µL | 91.008 µL | 182.015 µL | 910.075 µL | 1.82 mL |
10 mM | 9.101 µL | 45.504 µL | 91.008 µL | 455.038 µL | 910.075 µL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Citations
View all 11 citations: Citation Explorer
NMR reveals a dynamic allosteric pathway in thrombin
Authors: H, undefined and ley, L. D., Fuglestad, B., Stearns, K., Tonelli, M., Fenwick, R. B., Markwick, P. R., Komives, E. A.
Journal: Sci Rep (2017): 39575
Authors: H, undefined and ley, L. D., Fuglestad, B., Stearns, K., Tonelli, M., Fenwick, R. B., Markwick, P. R., Komives, E. A.
Journal: Sci Rep (2017): 39575
Continuous-flow injection microfluidic thrombin assays: The effect of binding kinetics on observed enzyme inhibition
Authors: Ramjee, M. K., Patel, S.
Journal: Anal Biochem (2017): 38-46
Authors: Ramjee, M. K., Patel, S.
Journal: Anal Biochem (2017): 38-46
Inherited Anti-Thrombin Deficiency in A Malay-Malaysian Family: A Missense Mutation at Nucleotide g.13267C>A aka anti-thrombin Budapest 5 (p.Pro439Thr) of the SERPINC 1 gene
Authors: Norlelawati, A. T., Rusmawati, I., Naznin, M., Nur Nadia, O., Rizqan Aizzani, R., Noraziana, A. W.
Journal: Med J Malaysia (2014): 27-30
Authors: Norlelawati, A. T., Rusmawati, I., Naznin, M., Nur Nadia, O., Rizqan Aizzani, R., Noraziana, A. W.
Journal: Med J Malaysia (2014): 27-30
The role of protein S in the activation of thrombin activatable fibrinolysis inhibitor (TAFI) and regulation of fibrinolysis
Authors: Mosnier, L. O., Meijers, J. C., Bouma, B. N.
Journal: Thromb Haemost (2001): 1040-6
Authors: Mosnier, L. O., Meijers, J. C., Bouma, B. N.
Journal: Thromb Haemost (2001): 1040-6
Protein C inhibitor regulates the thrombin-thrombomodulin complex in the up- and down regulation of TAFI activation
Authors: Mosnier, L. O., Elisen, M. G., Bouma, B. N., Meijers, J. C.
Journal: Thromb Haemost (2001): 1057-64
Authors: Mosnier, L. O., Elisen, M. G., Bouma, B. N., Meijers, J. C.
Journal: Thromb Haemost (2001): 1057-64
Thrombin activatable fibrinolysis inhibitor (TAFI) does not inhibit in vitro thrombolysis by pharmacological concentrations of t-PA
Authors: Colucci, M., D'Aprile, A. M., Italia, A., Gresele, P., Morser, J., Semeraro, N.
Journal: Thromb Haemost (2001): 661-6
Authors: Colucci, M., D'Aprile, A. M., Italia, A., Gresele, P., Morser, J., Semeraro, N.
Journal: Thromb Haemost (2001): 661-6
Synergistic actions of a thrombin-derived synthetic peptide and a thrombin receptor-activating peptide in stimulating fibroblast mitogenesis
Authors: Hollenberg, M. D., Mokashi, S., Leblond, L., DiMaio, J.
Journal: J Cell Physiol (1996): 491-6
Authors: Hollenberg, M. D., Mokashi, S., Leblond, L., DiMaio, J.
Journal: J Cell Physiol (1996): 491-6
Efficient production of the C-terminal domain of secretory leukoprotease inhibitor as a thrombin-cleavable fusion protein in Escherichia coli
Authors: Masuda, K., Kamimura, T., Kanesaki, M., Ishii, K., Imaizumi, A., Sugiyama, T., Suzuki, Y., Ohtsuka, E.
Journal: Protein Eng (1996): 101-6
Authors: Masuda, K., Kamimura, T., Kanesaki, M., Ishii, K., Imaizumi, A., Sugiyama, T., Suzuki, Y., Ohtsuka, E.
Journal: Protein Eng (1996): 101-6
Activation of thrombin-inactivated single-chain urokinase-type plasminogen activator by dipeptidyl peptidase I (cathepsin C)
Authors: Naul, undefined and , U., Rijken, D. C.
Journal: Eur J Biochem (1994): 497-501
Authors: Naul, undefined and , U., Rijken, D. C.
Journal: Eur J Biochem (1994): 497-501
Amidinated thrombin: preparation and peptidase activity
Authors: Kang, E. P.
Journal: Thromb Res (1978): 177-80
Authors: Kang, E. P.
Journal: Thromb Res (1978): 177-80
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines