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Bis-Arg-rhodamine 110

This Bis Arg-rhodamine 110 is a fluorogenic aminopeptidase and endopeptidase substrate used for the detection of enzymatic activity of human alpha-thrombin. The non-fluorescent substrate generates a bright green fluorescent rhodamine product, and can be easily detected with the FITC filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bis-Arg-rhodamine 110 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM91.008 µL455.038 µL910.075 µL4.55 mL9.101 mL
5 mM18.202 µL91.008 µL182.015 µL910.075 µL1.82 mL
10 mM9.101 µL45.504 µL91.008 µL455.038 µL910.075 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Citations

View all 11 citations: Citation Explorer
Continuous-flow injection microfluidic thrombin assays: The effect of binding kinetics on observed enzyme inhibition
Authors: Ramjee, M. K., Patel, S.
Journal: Anal Biochem (2017): 38-46
NMR reveals a dynamic allosteric pathway in thrombin
Authors: H, undefined and ley, L. D., Fuglestad, B., Stearns, K., Tonelli, M., Fenwick, R. B., Markwick, P. R., Komives, E. A.
Journal: Sci Rep (2017): 39575
Inherited Anti-Thrombin Deficiency in A Malay-Malaysian Family: A Missense Mutation at Nucleotide g.13267C>A aka anti-thrombin Budapest 5 (p.Pro439Thr) of the SERPINC 1 gene
Authors: Norlelawati, A. T., Rusmawati, I., Naznin, M., Nur Nadia, O., Rizqan Aizzani, R., Noraziana, A. W.
Journal: Med J Malaysia (2014): 27-30
Thrombin activatable fibrinolysis inhibitor (TAFI) does not inhibit in vitro thrombolysis by pharmacological concentrations of t-PA
Authors: Colucci, M., D'Aprile, A. M., Italia, A., Gresele, P., Morser, J., Semeraro, N.
Journal: Thromb Haemost (2001): 661-6
Protein C inhibitor regulates the thrombin-thrombomodulin complex in the up- and down regulation of TAFI activation
Authors: Mosnier, L. O., Elisen, M. G., Bouma, B. N., Meijers, J. C.
Journal: Thromb Haemost (2001): 1057-64
Page updated on December 6, 2024

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Catalog Number13481
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Physical properties

Molecular weight

1098.81

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

500

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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