BOC-Leu-Gly-Arg-Aminoluciferin

This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. BOC-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13001	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.971 µL	609.853 µL	1.22 mL	6.099 mL	12.197 mL
5 mM	24.394 µL	121.971 µL	243.941 µL	1.22 mL	2.439 mL
10 mM	12.197 µL	60.985 µL	121.971 µL	609.853 µL	1.22 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	819.87 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins

Authors:

Harwood KR, Mofford DM, Reddy GR, Miller SC.

Journal:

Chem Biol (2011): 1649

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

Authors:

Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.

Journal:

Apoptosis (2011): 198

Aminoluciferins as functional bioluminogenic substrates of firefly luciferase

Authors:

Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.

Journal:

Chem Asian J (2011): 1800

Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin

Authors:

Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.

Journal:

Cell Death Differ (2010): 1003

Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase

Authors:

Reddy, G. R.; Thompson, W. C.; Miller, S. C.

Journal:

J Am Chem Soc (2010): 13586-7

Physical properties

Molecular weight	819.87
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	362
Emission (nm)	499

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025