BOC-Val-Pro-Arg-AMC

BOC-Val-Pro-Arg-AMC is a sensitive fluorogenic substrate for alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The Km values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 21 and 25 uM respectively. The Kcat values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 105 and 89 s-1 respectively. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13461	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	134.816 µL	674.082 µL	1.348 mL	6.741 mL	13.482 mL
5 mM	26.963 µL	134.816 µL	269.633 µL	1.348 mL	2.696 mL
10 mM	13.482 µL	67.408 µL	134.816 µL	674.082 µL	1.348 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	741.75 g/mol	=	mL	x	mM	=	mmol

References

View all 120 references: Citation Explorer

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model

Authors:

Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.

Journal:

Gynecol Oncol (2009): 616

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7

Authors:

Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.

Journal:

J Biomol Screen (2009): 1

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

Authors:

Nakata H, Ohtsuki T, Sisido M.

Journal:

Anal Biochem (2009): 121

Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes

Authors:

An L, Liu L, Wang S.

Journal:

Biomacromolecules (2009): 454

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells

Authors:

Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.

Journal:

Antimicrob Agents Chemother (2009): 728

Physical properties

Molecular weight	741.75
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025