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BOC-Val-Pro-Arg-Aminoluciferin

BOC-Val-Pro-Arg-Aminoluciferin is a sentive luminescent substrate for alpha-thrombin. The non-luminescent substrate generates a bright luminescent product aminoluciferin that can be easily detected with luciferase. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM118.216 µL591.079 µL1.182 mL5.911 mL11.822 mL
5 mM23.643 µL118.216 µL236.432 µL1.182 mL2.364 mL
10 mM11.822 µL59.108 µL118.216 µL591.079 µL1.182 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)
BOC-Val-Pro-Arg-AMC341441

Citations

View all 1 citations: Citation Explorer
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155

References

View all 16 references: Citation Explorer
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Page updated on October 14, 2024

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Catalog Number13002
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Physical properties

Molecular weight

845.91

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

18000

Excitation (nm)

362

Emission (nm)

499

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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