BOC-Val-Pro-Arg-Aminoluciferin

BOC-Val-Pro-Arg-Aminoluciferin is a sentive luminescent substrate for alpha-thrombin. The non-luminescent substrate generates a bright luminescent product aminoluciferin that can be easily detected with luciferase. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13002	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	118.216 µL	591.079 µL	1.182 mL	5.911 mL	11.822 mL
5 mM	23.643 µL	118.216 µL	236.432 µL	1.182 mL	2.364 mL
10 mM	11.822 µL	59.108 µL	118.216 µL	591.079 µL	1.182 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	845.91 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins

Authors:

Harwood KR, Mofford DM, Reddy GR, Miller SC.

Journal:

Chem Biol (2011): 1649

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

Authors:

Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.

Journal:

Apoptosis (2011): 198

Aminoluciferins as functional bioluminogenic substrates of firefly luciferase

Authors:

Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.

Journal:

Chem Asian J (2011): 1800

Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin

Authors:

Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.

Journal:

Cell Death Differ (2010): 1003

Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase

Authors:

Reddy, G. R.; Thompson, W. C.; Miller, S. C.

Journal:

J Am Chem Soc (2010): 13586-7

Physical properties

Molecular weight	845.91
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	362
Emission (nm)	499

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025