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Bodi Fluor™ 488 amine

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Physical properties
Molecular weight462.23
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
462.23
Bodi Fluor 488 amine is an excellent building block for developing fluorescent probes. Bodi Fluor 488 is the same fluorophore as BODIPY FL (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene FL). It is a popular lipophilic fluorescent dye molecule used in biology, chemistry, and materials science. BODI Fluor 488 has high fluorescence quantum yields, photostability, and brightness. When excited with light in the blue to green range (around 488 to 515 nanometers), it emits around 510 to 520 nanometers. Researchers and scientists use BODIPY FL for various purposes, such as labeling biomolecules (proteins, nucleic acids, etc.) for fluorescence microscopy, flow cytometry, and other imaging techniques. It can also be employed as a probe for sensing and detecting various analytes or as a fluorescent marker in chemical and biological assays.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM216.343 µL1.082 mL2.163 mL10.817 mL21.634 mL
5 mM43.269 µL216.343 µL432.685 µL2.163 mL4.327 mL
10 mM21.634 µL108.171 µL216.343 µL1.082 mL2.163 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*504510810000.0150.018
Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2*504510810000.0150.018

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References


View all 50 references: Citation Explorer
Engineering Metastability into a Virus-like Particle to Enable Triggered Dissociation.
Authors: Starr, Caleb A and Nair, Smita and Huang, Sheng-Yuan and Hagan, Michael F and Jacobson, Stephen C and Zlotnick, Adam
Journal: Journal of the American Chemical Society (2023): 2322-2331
Pore-like diffusion barriers in murine cardiac myocytes.
Authors: Deisl, Christine and Chung, Jay A and Hilgemann, Donald W
Journal: bioRxiv : the preprint server for biology (2023)
Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 Activity in the Mammalian Polyamine Transport System.
Authors: Houdou, Marine and Jacobs, Nathalie and Coene, Jonathan and Azfar, Mujahid and Vanhoutte, Roeland and Van den Haute, Chris and Eggermont, Jan and Daniëls, Veronique and Verhelst, Steven H L and Vangheluwe, Peter
Journal: Biomolecules (2023)
High sensitivity (zeptomole) detection of BODIPY-labelled heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts.
Authors: Maciej-Hulme, Marissa L and Leprince, Anaëlle C N and Lavin, Andre and Guimond, Scott E and Turnbull, Jeremy E and Pelletier, Julien and Yates, Edwin A and Powell, Andrew K and Skidmore, Mark A
Journal: Analytical methods : advancing methods and applications (2023): 1461-1469
Fluorescence Intensity-Based eIF2B's Guanine Nucleotide-Exchange Factor Activity Assay.
Authors: Sekine, Yusuke and Ron, David and Zyryanova, Alisa F
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 187-196
Fluorescence Anisotropy Assay with Guanine Nucleotides Provides Access to Functional Analysis of Gαi1 Proteins.
Authors: Pepanian, Anna and Sommerfeld, Paul and Kasprzyk, Renata and Kühl, Toni and Binbay, F Ayberk and Hauser, Christoph and Löser, Reik and Wodtke, Robert and Bednarczyk, Marcelina and Chrominski, Mikolaj and Kowalska, Joanna and Jemielity, Jacek and Imhof, Diana and Pietsch, Markus
Journal: Analytical chemistry (2022): 14410-14418
Effective Penetration of a Liposomal Formulation of Bleomycin through Ex-Vivo Skin Explants from Two Different Species.
Authors: Ferrari, Giulia and Pang, Lisa Y and De Moliner, Fabio and Vendrell, Marc and Reardon, Richard J M and Higgins, Andrew J and Chopra, Sunil and Argyle, David J
Journal: Cancers (2022)
Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
Authors: Santos-Coquillat, Ana and González, María Isabel and Clemente-Moragón, Agustín and González-Arjona, Mario and Albaladejo-García, Virginia and Peinado, Héctor and Muñoz, Javier and Ximénez Embún, Pilar and Ibañez, Borja and Oliver, Eduardo and Desco, Manuel and Salinas, Beatriz
Journal: Small (Weinheim an der Bergstrasse, Germany) (2022): e2105421
Differential routing and disposition of the long-chain saturated fatty acid palmitate in rodent vs human beta-cells.
Authors: Thomas, Patricia and Arden, Catherine and Corcoran, Jenna and Hacker, Christian and Welters, Hannah J and Morgan, Noel G
Journal: Nutrition & diabetes (2022): 22
Pirenzepine Binding Sites in the Brain of the Honeybee Apis mellifera: Localization and Involvement in Non-Associative Learning.
Authors: Messikh, Chaïma and Gauthier, Monique and Armengaud, Catherine
Journal: Insects (2022)