Bodi Fluor™ 576/589 acid (equivalent to BODIPY™ 576/589)

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	329.11
Solvent	DMSO

Spectral properties

Excitation (nm)	573
Emission (nm)	592

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

740775-14-8

Molecular weight

329.11

Excitation (nm)

573

Emission (nm)

592

Bodi Fluor™ 576/589 is chemically the same molecule to BODIPY™ 576/589 dye. It is a bright red fluorescent dye with high cell-permeability. It has a high extinction coefficient and fluorescence quantum yield. Its fluorescence is relatively insensitive to solvent polarity and pH change. In contrast to the highly water-soluble fluorophores such as iFluor® and Alexa Fluor® 488 dyes, Bodi Fluor dyes have unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds. Bodi Fluor 576/589 dye has a relatively long excited-state lifetime (typically 5 nanoseconds or longer), which is useful for fluorescence polarization-based assays and a large two-photon cross-section for multiphoton excitation.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 576/589 acid (equivalent to BODIPY™ 576/589) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	303.85 µL	1.519 mL	3.038 mL	15.192 mL	30.385 mL
5 mM	60.77 µL	303.85 µL	607.7 µL	3.038 mL	6.077 mL
10 mM	30.385 µL	151.925 µL	303.85 µL	1.519 mL	3.038 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	573
Emission (nm)	592

Images

Figure 1. Chemical structure for Bodi Fluor™ 576/589 acid (equivalent to BODIPY™ 576/589).

References

View all 50 references: Citation Explorer

Ruthenium(II) Conjugates of Boron-Dipyrromethene and Biotin for Targeted Photodynamic Therapy in Red Light.
Authors: Paul, Subhadeep and Kundu, Paramita and Bhattacharyya, Utso and Garai, Aditya and Maji, Ram Chandra and Kondaiah, Paturu and Chakravarty, Akhil R
Journal: Inorganic chemistry (2020): 913-924

Selective Detection and Visualization of Exogenous/endogenous Hypochlorous Acid in Living Cells using a BODIPY-based Red-emitting Fluorescent Probe.
Authors: Wang, Xianhui and Tao, Yuanfang and Zhang, Jian and Chen, Miao and Wang, Nannan and Ji, Xin and Zhao, Weili
Journal: Chemistry, an Asian journal (2020)

Red fluorescent pyrazoline-BODIPY nanoparticles for ultrafast and long-term bioimaging.
Authors: Zhang, Yuandong and Zheng, Xiaohua and Zhang, Liping and Yang, Zhiyu and Chen, Li and Wang, Lei and Liu, Shi and Xie, Zhigang
Journal: Organic & biomolecular chemistry (2020): 707-714

Long-Lived Triplet Excited State Accessed with Spin-Orbit Charge Transfer Intersystem Crossing in Red Light-Absorbing Phenoxazine-Styryl BODIPY Electron Donor/Acceptor Dyads.
Authors: Dong, Yu and Elmali, Ayhan and Zhao, Jianzhang and Dick, Bernhard and Karatay, Ahmet
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2020): 1388-1401

Red/NIR Thermally Activated Delayed Fluorescence from Aza-BODIPYs.
Authors: Avellanal-Zaballa, Edurne and Prieto-Castañeda, Alejandro and García-Garrido, Fernando and Agarrabeitia, Antonia R and Rebollar, Esther and Bañuelos, Jorge and García-Moreno, Inmaculada and Ortiz, María J
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Red-light-sensitive BODIPY photoprotecting groups for amines and their biological application in controlling heart rhythm.
Authors: Sitkowska, Kaja and Hoes, Martijn F and Lerch, Michael M and Lameijer, Lucien N and van der Meer, Peter and Szymański, Wiktor and Feringa, Ben L
Journal: Chemical communications (Cambridge, England) (2020): 5480-5483

Far-red photoactivatable BODIPYs for the super-resolution imaging of live cells.
Authors: Zhang, Yang and Tang, Sicheng and Ravelo, Laura and Cusido, Janet and Raymo, Françisco M
Journal: Methods in enzymology (2020): 131-147

Mechanoresponsive Behavior of a Polymer-Embedded Red-Light Emitting Rotaxane Mechanophore.
Authors: Muramatsu, Tatsuya and Sagara, Yoshimitsu and Traeger, Hanna and Tamaoki, Nobuyuki and Weder, Christoph
Journal: ACS applied materials & interfaces (2019): 24571-24576

Pyrrolopyrrole Aza-BODIPY Analogues as Near-Infrared Chromophores and Fluorophores: Red-Shift Effects of Substituents on Absorption and Emission Spectra.
Authors: Kage, Yuto and Karasaki, Hideaki and Mori, Shigeki and Furuta, Hiroyuki and Shimizu, Soji
Journal: ChemPlusChem (2019): 1648-1652

Lysosome-targeting turn-on red/NIR BODIPY probes for imaging hypoxic cells.
Authors: Kong, Xiangduo and Di, Linting and Fan, Yunshi and Zhou, Zhikuan and Feng, Xinjiang and Gai, Lizhi and Tian, Jiangwei and Lu, Hua
Journal: Chemical communications (Cambridge, England) (2019): 11567-11570