BTC, AM *CAS 176767-94-5*

BTC, AM

CAS 176767-94-5

This cell-permeant coumarin Ca2+ indicator BTC, AM exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, enabling ratiometric calcium measurements. Due to its high selectivity and low affinity for Ca2+ (Kd ~7 uM) BTC is often used for the quantitation of high intracellular Ca2+levels. In addition, BTC, AM has also been used for monitoring potassium channel since thallium ion enhances the fluorescence of BTC.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21054	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BTC, AM *CAS 176767-94-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.049 µL	510.246 µL	1.02 mL	5.102 mL	10.205 mL
5 mM	20.41 µL	102.049 µL	204.098 µL	1.02 mL	2.041 mL
10 mM	10.205 µL	51.025 µL	102.049 µL	510.246 µL	1.02 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	979.92 g/mol	=	mL	x	mM	=	mmol

Physical properties

Dissociation constant (K_d, nM)	7000
Molecular weight	979.92
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	176767-94-5

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	400, 480
Emission	540
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Page updated on October 12, 2025