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BTC, tetrapotassium salt *CAS 216453-54-2*

This cell-impermeant coumarin Ca2+ indicator exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, enabling ratiometric calcium measurements. Due to its high selectivity and low affinity for Ca2+ (Kd ~7 uM) BTC is often used for the quantitation of high Ca2+levels.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of BTC, tetrapotassium salt *CAS 216453-54-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM118.481 µL592.403 µL1.185 mL5.924 mL11.848 mL
5 mM23.696 µL118.481 µL236.961 µL1.185 mL2.37 mL
10 mM11.848 µL59.24 µL118.481 µL592.403 µL1.185 mL

Molarity calculator

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Citations

References

View all 1 references: Citation Explorer
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
Page updated on December 6, 2024

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Physical properties

Dissociation constant (Kd, nM)7000

Molecular weight

844.02

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

216453-54-2
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