BTC, tetrapotassium salt *CAS 216453-54-2*

BTC, tetrapotassium salt

CAS 216453-54-2

This cell-impermeant coumarin Ca2+ indicator exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, enabling ratiometric calcium measurements. Due to its high selectivity and low affinity for Ca2+ (Kd ~7 uM) BTC is often used for the quantitation of high Ca2+levels.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21053	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of BTC, tetrapotassium salt *CAS 216453-54-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	118.481 µL	592.403 µL	1.185 mL	5.924 mL	11.848 mL
5 mM	23.696 µL	118.481 µL	236.961 µL	1.185 mL	2.37 mL
10 mM	11.848 µL	59.24 µL	118.481 µL	592.403 µL	1.185 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	844.02 g/mol	=	mL	x	mM	=	mmol

Physical properties

Dissociation constant (K_d, nM)	7000
Molecular weight	844.02
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	216453-54-2

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Page updated on October 12, 2025