BTC, tetrapotassium salt CAS 216453-54-2

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Physical properties

Dissociation constant (K_d, nM)	7000
Molecular weight	844.02
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

216453-54-2

Molecular weight

844.02

Dissociation constant (K_d, nM)

7000

This cell-impermeant coumarin Ca2+ indicator exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, enabling ratiometric calcium measurements. Due to its high selectivity and low affinity for Ca2+ (Kd ~7 uM) BTC is often used for the quantitation of high Ca2+levels.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of BTC, tetrapotassium salt *CAS 216453-54-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	118.481 µL	592.403 µL	1.185 mL	5.924 mL	11.848 mL
5 mM	23.696 µL	118.481 µL	236.961 µL	1.185 mL	2.37 mL
10 mM	11.848 µL	59.24 µL	118.481 µL	592.403 µL	1.185 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for BTC, tetrapotassium salt *CAS 216453-54-2*

Citations

View all 1 citations: Citation Explorer

Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
Authors: Weaver, C David
Journal: (2018): 105--114

References

View all 1 references: Citation Explorer

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75