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Bucculite™ FdU Cu-Free Cell Proliferation Fluorescence Imaging Kit
Green Fluorescence
Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite™ FdU Cu-Free Cell Proliferation Fluorescence Imaging Kit uses FOL-FdU, an analog of thymidine. FOL-FdU is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated FOL-FdU is labelled with MTA-iFluor® 488 through our Buccutite™ labeling technology. The resulted iFluor® 488-labeled DNA formed in cells is visualized in FITC Channel. Bucculite™ FdU Cu-Free Cell Proliferation Fluorescence Imaging Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry assay. It is an environment friendly copper-free assay for measuring active DNA synthesis at single-cell level.
S-phase HeLa cells were detected with Bucculite™ FdU-Cu Free Cell Proliferation Fluorescence Imaging Kit (Cat#22305). HeLa cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with FOL-FdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 488-MTA for 30min in staining buffer, and then washed three times with 1X washing Buffer. 100µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the fluorescence images were visualized with FITC filter for S phase cells (Green) and with DAPI filter nuclear for all cells (Blue).
S-phase HeLa cells were detected with Bucculite™ FdU-Cu Free Cell Proliferation Fluorescence Imaging Kit (Cat#22305). HeLa cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with FOL-FdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 488-MTA for 30min in staining buffer, and then washed three times with 1X washing Buffer. 100µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the fluorescence images were visualized with FITC filter for S phase cells (Green) and with DAPI filter nuclear for all cells (Blue).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22305200 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation490 nm
Emission525 nm
Recommended plateBlack wall/clear bottom
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Page updated on October 14, 2025