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Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit
Red Laser-Comptatible
Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with iFluor® 647 MTA. The resulted iFluor® 647-labeled DNA formed in cells is visualized in Cy5 Channel. Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.
S-phase Jurkat cells were detected with Bucculite™ XdU Cell Proliferation Flow Cytometry Kit (Cat#22325). Jurkat cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 647-MTA for 30 min in staining buffer, and then washed three times with 1X washing Buffer. 100 µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the flow cytometry was performed using flow cytometry with APC channel.
S-phase Jurkat cells were detected with Bucculite™ XdU Cell Proliferation Flow Cytometry Kit (Cat#22325). Jurkat cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 647-MTA for 30 min in staining buffer, and then washed three times with 1X washing Buffer. 100 µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the flow cytometry was performed using flow cytometry with APC channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22325100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation633/640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025