Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit provides a fast and mild method for analyzing active DNA synthesis in proliferating cells by flow cytometry. The assay detects XdU incorporation into newly synthesized DNA, enabling direct measurement of cells undergoing S-phase progression without the harsh DNA denaturation steps required for traditional BrdU-based detection.

The kit is designed for multicolor flow cytometry workflows and uses chemistry developed to minimize copper-related quenching of fluorescent proteins and phycobiliprotein-based fluorophores such as R-PE and PE-tandems. Its streamlined workflow avoids lengthy antibody incubation steps and supports completion of labeling and detection in as little as 60 minutes. Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit is suitable for cell proliferation analysis, S-phase quantification, compound response studies, and multiplex flow cytometry applications.