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Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit
Violet Laser-Compatible
Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with mFluor™ Violet 450 MTA. The resulted mFluor™ Violet 450-labeled DNA formed in cells is visualized in Pacific Blue Channel. Bucculite™ Flow Cytometric XdU Cell Proliferation Assay Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.
The flow cytometric response of S-phase Jurkat cells detected with Buccutite™ XdU Cell Proliferation flow cytometry kit (Cat#22321). Jurkat cells were seeded at 50,000 cells/well/100 μL overnight in a 6-black wall plate. Cells were treated with XdU at 37 ºC for 3 hours, fixed and permeabilized as per protocol. Cells were then stained with mFluor™ Violet 450-azide for 30 mins in staining buffer, and washed three times with PBS. The fluorescence response was measured with NovoCyte flow cytometer using Pacific Blue channel.
The flow cytometric response of S-phase Jurkat cells detected with Buccutite™ XdU Cell Proliferation flow cytometry kit (Cat#22321). Jurkat cells were seeded at 50,000 cells/well/100 μL overnight in a 6-black wall plate. Cells were treated with XdU at 37 ºC for 3 hours, fixed and permeabilized as per protocol. Cells were then stained with mFluor™ Violet 450-azide for 30 mins in staining buffer, and washed three times with PBS. The fluorescence response was measured with NovoCyte flow cytometer using Pacific Blue channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22321100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 19, 2025