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Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit
Green Fluorescence
Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with iFluor® 488 MTA. The resulted iFluor® 488-labeled DNA formed in cells is imaged with a FITC filter set. Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry-based assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.
S-phase HeLa cells were detected with Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit (Cat#22326). HeLa cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 488-MTA for 30 min in staining buffer, and then washed three times with 1X washing Buffer. 100µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the fluorescence images were visualized with FITC filter for S phase cells (Green) and with DAPI filter nuclear for all cells (Blue).
S-phase HeLa cells were detected with Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit (Cat#22326). HeLa cells at 50,000 cells/well/100 μL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37 ºC for 3 hours, and fixed with Methanol/PBS (90/10).  After fixation, cells were stained with iFluor® 488-MTA for 30 min in staining buffer, and then washed three times with 1X washing Buffer. 100µL 5 µg/ml Hoechst 33342 solution in 1X Washing Buffer were added to each well and the fluorescence images were visualized with FITC filter for S phase cells (Green) and with DAPI filter nuclear for all cells (Blue).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22326200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 14, 2025