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Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit
Red Fluorescence
Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with iFluor® 555 MTA. The resulted iFluor® 555-labeled DNA formed in cells is imaged with a Cy3 filter set. Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry-based assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.
S-phase HeLa cells detection using the Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit. HeLa cells (50,000 cells/well in 100 µL) were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37°C for 3 hours, then fixed with Methanol/PBS (90/10). After fixation, cells were stained with iFluor® 555-MTA for 30 minutes in staining buffer, followed by three washes with 1X Washing Buffer. Nuclear staining was performed with 100 µL of 5 µg/mL Hoechst 33342 solution in 1X Washing Buffer. Fluorescence images were acquired using a TRITC filter to visualize S-phase cells (red) and a DAPI filter for nuclear staining (blue).
S-phase HeLa cells detection using the Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit. HeLa cells (50,000 cells/well in 100 µL) were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with XdU at 37°C for 3 hours, then fixed with Methanol/PBS (90/10). After fixation, cells were stained with iFluor® 555-MTA for 30 minutes in staining buffer, followed by three washes with 1X Washing Buffer. Nuclear staining was performed with 100 µL of 5 µg/mL Hoechst 33342 solution in 1X Washing Buffer. Fluorescence images were acquired using a TRITC filter to visualize S-phase cells (red) and a DAPI filter for nuclear staining (blue).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22327200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 17, 2025