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Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kit
Optimized for Labeling 100 ug Protein
Protein-protein conjugations are commonly performed with a bifunctional linker (such as SMCC) having different reactivity at each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) of lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) of cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins contain multiple amine and thiol groups that cause significant amount of crosslinkings. In addition, it is quite difficult and tedious to quantify the number of maleimide groups in a protein. Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kit is designed to rapidly prepare ALP conjugates with pre-activated ALP. The kit is optimized for labeling 100 ug protein. The Buccutite™ FOL-activated ALP readily reacts with Buccutite™ MTA-modified antibody under extremely mild neutral conditions without any catalyst required. Compared with commonly used SMCC and other similar technologies, Buccutite™ bioconjugation system is much more robust and easier to use. It enables faster and quantitative conjugation of biomolecules with higher efficiency and yields.
Mouse IgG dose curve was detected using the ALP- goat-anti-mouse IgG conjugate prepared with Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kit. 3-fold serial diluted mouse IgG was coated on a 96-well plate, and 100uL GAM IgG-ALP conjugate (1ug/ml) was added to each well followed the standard ELISA method. pNPP substrate solution was used to detect the immobilized mouse IgG with 30 min incubation and read at 400 nm.
Mouse IgG dose curve was detected using the ALP- goat-anti-mouse IgG conjugate prepared with Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kit. 3-fold serial diluted mouse IgG was coated on a 96-well plate, and 100uL GAM IgG-ALP conjugate (1ug/ml) was added to each well followed the standard ELISA method. pNPP substrate solution was used to detect the immobilized mouse IgG with 30 min incubation and read at 400 nm.
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55132 Labelings
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on September 25, 2025