Buccutite™ FOL, maleimide [FOLM]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 523.63 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Buccutite™ Crosslinkers and Kits
Molecular weight 523.63 |
Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ FOL maleimide (FOLM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the FOLM reacts (via maleimide) with thiols (-SH) of cysteineS found in the reduced antibodies (by TCEP or DTT) to give FOL-modified reduced antibodies. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required while the SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking is robust and efficient.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ FOL, maleimide [FOLM] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 190.975 µL | 954.873 µL | 1.91 mL | 9.549 mL | 19.097 mL |
5 mM | 38.195 µL | 190.975 µL | 381.949 µL | 1.91 mL | 3.819 mL |
10 mM | 19.097 µL | 95.487 µL | 190.975 µL | 954.873 µL | 1.91 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 21 references: Citation Explorer
N-Hydroxysuccinimide-Modified Ethynylphosphonamidates Enable the Synthesis of Configurationally Defined Protein Conjugates.
Authors: Kasper, Marc-André and Gerlach, Marcus and Schneider, Anselm F L and Groneberg, Christiane and Ochtrop, Philipp and Boldt, Stefanie and Schumacher, Dominik and Helma, Jonas and Leonhardt, Heinrich and Christmann, Mathias and Hackenberger, Christian P R
Journal: Chembiochem : a European journal of chemical biology (2020): 113-119
Authors: Kasper, Marc-André and Gerlach, Marcus and Schneider, Anselm F L and Groneberg, Christiane and Ochtrop, Philipp and Boldt, Stefanie and Schumacher, Dominik and Helma, Jonas and Leonhardt, Heinrich and Christmann, Mathias and Hackenberger, Christian P R
Journal: Chembiochem : a European journal of chemical biology (2020): 113-119
MicroSPECT/CT Imaging of Cell-Line and Patient-Derived EGFR-Positive Tumor Xenografts in Mice with Panitumumab Fab Modified with Hexahistidine Peptides To Enable Labeling with 99mTc(I) Tricarbonyl Complex.
Authors: Ku, Anthony and Chan, Conrad and Aghevlian, Sadaf and Cai, Zhongli and Cescon, David and Bratman, Scott V and Ailles, Laurie and Hedley, David W and Reilly, Raymond M
Journal: Molecular pharmaceutics (2019): 3559-3568
Authors: Ku, Anthony and Chan, Conrad and Aghevlian, Sadaf and Cai, Zhongli and Cescon, David and Bratman, Scott V and Ailles, Laurie and Hedley, David W and Reilly, Raymond M
Journal: Molecular pharmaceutics (2019): 3559-3568
Understanding How the Stability of the Thiol-Maleimide Linkage Impacts the Pharmacokinetics of Lysine-Linked Antibody-Maytansinoid Conjugates.
Authors: Ponte, Jose F and Sun, Xiuxia and Yoder, Nicholas C and Fishkin, Nathan and Laleau, Rassol and Coccia, Jennifer and Lanieri, Leanne and Bogalhas, Megan and Wang, Lintao and Wilhelm, Sharon and Widdison, Wayne and Pinkas, Jan and Keating, Thomas A and Chari, Ravi and Erickson, Hans K and Lambert, John M
Journal: Bioconjugate chemistry (2016): 1588-98
Authors: Ponte, Jose F and Sun, Xiuxia and Yoder, Nicholas C and Fishkin, Nathan and Laleau, Rassol and Coccia, Jennifer and Lanieri, Leanne and Bogalhas, Megan and Wang, Lintao and Wilhelm, Sharon and Widdison, Wayne and Pinkas, Jan and Keating, Thomas A and Chari, Ravi and Erickson, Hans K and Lambert, John M
Journal: Bioconjugate chemistry (2016): 1588-98
2-(Maleimidomethyl)-1,3-Dioxanes (MD): a Serum-Stable Self-hydrolysable Hydrophilic Alternative to Classical Maleimide Conjugation.
Authors: Dovgan, Igor and Kolodych, Sergii and Koniev, Oleksandr and Wagner, Alain
Journal: Scientific reports (2016): 30835
Authors: Dovgan, Igor and Kolodych, Sergii and Koniev, Oleksandr and Wagner, Alain
Journal: Scientific reports (2016): 30835
Preparation and characterization of albumin conjugates of a truncated peptide YY analogue for half-life extension.
Authors: Ehrlich, George K and Michel, Hanspeter and Truitt, Theresa and Riboulet, William and Pop-Damkov, Petar and Goelzer, Petra and Hainzl, Dominik and Qureshi, Farooq and Lueckel, Barbara and Danho, Waleed and Conde-Knape, Karin and Konkar, Anish
Journal: Bioconjugate chemistry (2013): 2015-24
Authors: Ehrlich, George K and Michel, Hanspeter and Truitt, Theresa and Riboulet, William and Pop-Damkov, Petar and Goelzer, Petra and Hainzl, Dominik and Qureshi, Farooq and Lueckel, Barbara and Danho, Waleed and Conde-Knape, Karin and Konkar, Anish
Journal: Bioconjugate chemistry (2013): 2015-24
Enhanced extravasation, stability and in vivo cardiac gene silencing via in situ siRNA-albumin conjugation.
Authors: Lau, Shannen and Graham, Bim and Cao, Nga and Boyd, Ben J and Pouton, Colin W and White, Paul J
Journal: Molecular pharmaceutics (2012): 71-80
Authors: Lau, Shannen and Graham, Bim and Cao, Nga and Boyd, Ben J and Pouton, Colin W and White, Paul J
Journal: Molecular pharmaceutics (2012): 71-80
Protein labeling with the labeling precursor [(18)F]SiFA-SH for positron emission tomography.
Authors: Wängler, Björn and Kostikov, Alexey P and Niedermoser, Sabrina and Chin, Joshua and Orchowski, Katy and Schirrmacher, Esther and Iovkova-Berends, Liuba and Jurkschat, Klaus and Wängler, Carmen and Schirrmacher, Ralf
Journal: Nature protocols (2012): 1964-9
Authors: Wängler, Björn and Kostikov, Alexey P and Niedermoser, Sabrina and Chin, Joshua and Orchowski, Katy and Schirrmacher, Esther and Iovkova-Berends, Liuba and Jurkschat, Klaus and Wängler, Carmen and Schirrmacher, Ralf
Journal: Nature protocols (2012): 1964-9
Commercially Supplied Amine-Modified siRNAs May Require Ultrafiltration prior to Conjugation with Amine-Reactive Compounds.
Authors: Lau, Shannen and Graham, Bim and Boyd, Ben J and Pouton, Colin W and White, Paul J
Journal: Journal of nucleic acids (2011): 154609
Authors: Lau, Shannen and Graham, Bim and Boyd, Ben J and Pouton, Colin W and White, Paul J
Journal: Journal of nucleic acids (2011): 154609
Comparison of hydrazone heterobifunctional cross-linking agents for reversible conjugation of thiol-containing chemistry.
Authors: Christie, R James and Anderson, Diana J and Grainger, David W
Journal: Bioconjugate chemistry (2010): 1779-87
Authors: Christie, R James and Anderson, Diana J and Grainger, David W
Journal: Bioconjugate chemistry (2010): 1779-87
Synthesis, characterization and in vitro evaluation of a bone targeting delivery system for salmon calcitonin.
Authors: Bhandari, Krishna Hari and Newa, Madhuri and Uludag, Hasan and Doschak, Michael R
Journal: International journal of pharmaceutics (2010): 26-34
Authors: Bhandari, Krishna Hari and Newa, Madhuri and Uludag, Hasan and Doschak, Michael R
Journal: International journal of pharmaceutics (2010): 26-34
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?