Name: Buccutite™ FOL, maleimide [FOLM]
Brand: AAT Bioquest
SKU: 5356
Price: 213 USD
Availability: InStock

Buccutite™ FOL, maleimide

FOLM

Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ FOL maleimide (FOLM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the FOLM reacts (via maleimide) with thiols (-SH) of cysteineS found in the reduced antibodies (by TCEP or DTT) to give FOL-modified reduced antibodies. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required while the SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking is robust and efficient.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5356	2 umoles	Price

References

View all 21 references: Citation Explorer

N-Hydroxysuccinimide-Modified Ethynylphosphonamidates Enable the Synthesis of Configurationally Defined Protein Conjugates.

Authors:

Kasper, Marc-André and Gerlach, Marcus and Schneider, Anselm F L and Groneberg, Christiane and Ochtrop, Philipp and Boldt, Stefanie and Schumacher, Dominik and Helma, Jonas and Leonhardt, Heinrich and Christmann, Mathias and Hackenberger, Christian P R

Journal:

Chembiochem : a European journal of chemical biology (2020): 113-119

MicroSPECT/CT Imaging of Cell-Line and Patient-Derived EGFR-Positive Tumor Xenografts in Mice with Panitumumab Fab Modified with Hexahistidine Peptides To Enable Labeling with 99mTc(I) Tricarbonyl Complex.

Authors:

Ku, Anthony and Chan, Conrad and Aghevlian, Sadaf and Cai, Zhongli and Cescon, David and Bratman, Scott V and Ailles, Laurie and Hedley, David W and Reilly, Raymond M

Journal:

Molecular pharmaceutics (2019): 3559-3568

Understanding How the Stability of the Thiol-Maleimide Linkage Impacts the Pharmacokinetics of Lysine-Linked Antibody-Maytansinoid Conjugates.

Authors:

Ponte, Jose F and Sun, Xiuxia and Yoder, Nicholas C and Fishkin, Nathan and Laleau, Rassol and Coccia, Jennifer and Lanieri, Leanne and Bogalhas, Megan and Wang, Lintao and Wilhelm, Sharon and Widdison, Wayne and Pinkas, Jan and Keating, Thomas A and Chari, Ravi and Erickson, Hans K and Lambert, John M

Journal:

Bioconjugate chemistry (2016): 1588-98

2-(Maleimidomethyl)-1,3-Dioxanes (MD): a Serum-Stable Self-hydrolysable Hydrophilic Alternative to Classical Maleimide Conjugation.

Authors:

Dovgan, Igor and Kolodych, Sergii and Koniev, Oleksandr and Wagner, Alain

Journal:

Scientific reports (2016): 30835

Preparation and characterization of albumin conjugates of a truncated peptide YY analogue for half-life extension.

Authors:

Ehrlich, George K and Michel, Hanspeter and Truitt, Theresa and Riboulet, William and Pop-Damkov, Petar and Goelzer, Petra and Hainzl, Dominik and Qureshi, Farooq and Lueckel, Barbara and Danho, Waleed and Conde-Knape, Karin and Konkar, Anish

Journal:

Bioconjugate chemistry (2013): 2015-24

Physical properties

Molecular weight	523.63
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025