Buccutite™ FOL, NHS ester

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite™ MTA and Molecule-2-Buccutite™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient. A number of our customers have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC. One end of the SMCC reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition, it is quite difficult and tedious to quantify the number of maleimide groups on a protein.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5350	2 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ FOL, NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	115.342 µL	576.708 µL	1.153 mL	5.767 mL	11.534 mL
5 mM	23.068 µL	115.342 µL	230.683 µL	1.153 mL	2.307 mL
10 mM	11.534 µL	57.671 µL	115.342 µL	576.708 µL	1.153 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	866.99 g/mol	=	mL	x	mM	=	mmol

Citations

View all 10 citations: Citation Explorer

Identification of pyridinoline, a collagen crosslink, as a novel intrinsic ligand for the receptor for advanced glycation end-products (RAGE)

Authors:

Murakami, Y., Fujino, T., Kurachi, R., Hasegawa, T., Usui, T., Hayase, F., Watanabe, H.

Journal:

Biosci Biotechnol Biochem (2018): 1508-1514

Structure-based non-canonical amino acid design to covalently crosslink an antibody-antigen complex

Authors:

Xu, J., Tack, D., Hughes, R. A., Ellington, A. D., Gray, J. J.

Journal:

J Struct Biol (2014): 215-22

Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications

Authors:

Vashist, S. K.

Journal:

Diagnostics (Basel) (2012): 23-33

Promotion of the activity of Akt in CD8+ T cells by the crosslink of 3H3, an agonist antibody of 4-1BB

Authors:

Yang, Z., Fu, X. L., Shang, X. Y., Niu, W., Wu, Y. Z., Wang, L.

Journal:

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2007): 223-5

Requirement for PCNA and RPA in interstrand crosslink-induced DNA synthesis

Authors:

Li, L., Peterson, C. A., Zhang, X., Legerski, R. J.

Journal:

Nucleic Acids Res (2000): 1424-7

Physical properties

Molecular weight	866.99
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 7, 2025